HAX1 antibody | AB03/4C6

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Mouse anti HAX1

Product Type
PrecisionAb Monoclonal
Clone
AB03/4C6
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00915
Datasheet
IP WB
SDS
100 µl loader

Mouse anti HAX1 antibody recognizes HCLS1-associated protein X-1, also known as HSP1BP-1.

The ubiquitously-expressed protein HAX1 has functions in control of cell viability, migration, and cancer progression, and has been reported in the mitochondria and cytoplasm (Li et al. 2016). Mutations in HAX1 cause severe congenital neutropenia (SCN), a condition characterised by life-threatening bacterial infections and a dramatic decrease in peripheral neutrophil granulocytes (Klein 2017). Transcription of the gene encoding HAX1 is activated by the histone demethylase KDM4B, which show correlated overexpression in colorectal cancer cells and play a role in mitochondrial apoptosis (Li et al. 2016). HAX1 may play an important role in the invasion and metastasis of breast cancer, has been suggested as a potential therapeutic target (Sheng and Ni 2015).

Product Details

Target Species
Human
Western Blotting
Mouse anti HAX1 detects a band of approximately 32 kDa in Jurkat cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-279 of human HAX1
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O00165
Entrez Gene
HAX1
GO Terms
GO:0005739 mitochondrion
GO:0005625 soluble fraction
GO:0015629 actin cytoskeleton
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0016529 sarcoplasmic reticulum
GO:0019966 interleukin-1 binding
GO:0030027 lamellipodium
GO:0031965 nuclear membrane
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of HAX1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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