Haptoglobin antibody

100% Secure


Mouse anti Haptoglobin

Haptoglobin antibody recognizes the haptoglobin protein, also known as haptoglobin alpha (1S)-beta, haptoglobin alpha (2FS)-beta, liver regeneration-related protein LRRG173 or zonulin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00089 WB 100 µl
Mouse anti Human haptoglobin antibody recognizes haptoglobin, one of the major acute phase plasma proteins (APP).

The haptoglobin gene (HP) encodes a preproprotein, also known as zonulin, which is processed to yield both alpha and beta chains. These chains subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin.

Mutations in HP and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. HP has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to HP on chromosome 16. Multiple transcript variants encoding different isoforms have been found for HP (provided by RefSeq, Jul 2008).

Mouse anti Human haptoglobin antibody detects a band of 43 kDa. The antibody has been extensively validated for western blotting using human plasma lysates.

Product Details

Target Species
Human
Western Blotting
Anti haptoglobin detects a band of approximately 43 kDa in human plasma samples.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified protein from human plasma
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P00738 Related reagents
Entrez Gene
HP Related reagents
GO Terms
GO:0006952 defense response
GO:0004252 serine-type endopeptidase activity
GO:0005576 extracellular region
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0010942 positive regulation of cell death
GO:0030492 hemoglobin binding
GO:0051354 negative regulation of oxidoreductase activity
GO:2000296 negative regulation of hydrogen peroxide catabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Haptoglobin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted