GORASP1 antibody | OTI5G8
Mouse anti GORASP1
GORASP1 antibody recognizes golgi reassembly stacking protein 1, also known as golgi peripheral membrane protein p65 or golgi phosphoprotein 5. This antibody has been extensively validated for western blotting using whole cell lysates.
GORASP1 Antibody with Control Lysate
GORASP1 antibody recognizes golgi reassembly stacking protein 1, also known as golgi peripheral membrane protein p65 or golgi phosphoprotein 5. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI5G8
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00136 | WB | 100 µl | ||||
| VMA00136KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human GORASP1 antibody recognizes the Golgi reassembly-stacking protein 1 (GORASP1), also known as Golgi peripheral membrane protein p65 and Golgi phosphoprotein 5.
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. Encoded by the GORASP1 gene, the Golgi reassembly-stacking protein 1 is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of GORASP1 (provided by RefSeq, Jul 2013).
Mouse anti Human GORASP1 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. Encoded by the GORASP1 gene, the Golgi reassembly-stacking protein 1 is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of GORASP1 (provided by RefSeq, Jul 2013).
Mouse anti Human GORASP1 antibody detects a band of 72 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti GORASP1 detects a band of approximately 72 kDa in HEK293 cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human GORASP1 (NP_114105) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q9BQQ3 Related reagents
- Entrez Gene
- GORASP1 Related reagents
- GO Terms
- GO:0000088 mitotic prophase
- GO:0016020 membrane
- GO:0005829 cytosol
- GO:0005794 Golgi apparatus
- GO:0015031 protein transport
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of GORASP1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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