GNL3 antibody | AB03/2F7

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Mouse anti GNL3

Product Type
PrecisionAb Monoclonal
Clone
AB03/2F7
Isotype
IgG2b
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00887
Datasheet
WB
SDS
100 µl loader

Mouse anti GNL3 antibody recognizes guanine nucleotide-binding protein-like 3, also known as NNP47, E2IG3, NS, and nucleostemin.

Nucleostemin is a nucleolar protein, but rapidly shuttles between the nucleolar and nucleoplasmic compartments when bound to GTP, and this shuttling is regulated by multiple control mechanisms (Kavyasudha et al. 2018). It appears to play a role in protecting DNA damage, both on the telomeres and non-telomeric regions of the chromosomes. It is highly expressed in various types of stem cells, tumors, and cancer stem cells, and is expressed at a much lower level in differentiated cells (Tsai et al. 2014), appearing to play a role in the cell cycle and proliferation of rapidly-dividing cells (Kavyasudha et al. 2018). Accordingly, nucleostemin contributes to formation of blastocysts, embryogenesis, regeneration of postnatal tissue, reprogramming of cells to pluripotency, and cancer development (Tsai et al. 2014). Nucleostemin is highly expressed in a wide variety of cancers, and could serve as a marker for tumor progression and poor patient outcome (Sami et al. 2019).

Product Details

Target Species
Human
Western Blotting
Mouse anti GNL3 antibody detects a band of approximately 65 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-226 of human GNL3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q9BVP2
Entrez Gene
GNL3
GO Terms
GO:0005515 protein binding
GO:0005525 GTP binding
GO:0005730 nucleolus
GO:0042127 regulation of cell proliferation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of GNL3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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