GNB3 antibody | EF01/1A12

100% Secure


Mouse anti GNB3

Product Type
PrecisionAb Monoclonal
Clone
EF01/1A12
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00948
Datasheet
WB
SDS
100 µl loader

Mouse anti GNB3 antibody recognizes guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, also known as transducin beta chain 3. GNB3 is the beta3 subunit of G-protein. G proteins transmit information from G-protein-coupled receptors at the plasma membrane to intracellular signaling pathways, regulating a variety of biochemical processes (Syrovatkina et al. 2016) including cell growth and mitosis (Wang and Zhang 2014). G-proteins, and also GNB3, appear to be associated with obesity. Duplication of the gene encoding GNB3 has been linked to an obesity phenotype in humans and in mice (Li et al. 2016). The C825T polymorphism (rs5443) of GNB3 has been associated with obesity, essential hypertension, atherosclerosis, coronary diseases, and cerebrovascular events with sex-specific effects (Gbadoe et al. 2016).

Product Details

Target Species
Human
Western Blotting
Mouse anti GNB3 detects a band of approximately 32 kDa in mouse brain tissue lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-340 of human GNB3
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P16520
Entrez Gene
GNB3
GO Terms
GO:0005886 plasma membrane
GO:0003924 GTPase activity
GO:0004871 signal transducer activity
GO:0006112 energy reserve metabolic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0007268 synaptic transmission
GO:0008217 regulation of blood pressure
GO:0071377 cellular response to glucagon stimulus
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of GNB3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra