GMNN antibody

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Mouse anti GMNN

GMNN antibody recognizes geminin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
VMA00332 Datasheet	WB MSDS	100 µl	Log in		Get Quote

Summary
Secondary Antibodies

Mouse anti Human GMNN antibody recognizes geminin.

Encoded by the GMNN gene, geminin plays a critical role in cell cycle regulation, inhibiting DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. Geminin is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of GMNN may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for GMNN, and two pseudogenes of GMNN are located on the short arm of chromosome 16 (provided by RefSeq, Oct 2011).

Mouse anti Human GMNN antibody detects a band of 30 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti GMNN detects a band of approximately 30 kDa in K562 cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human GMNN
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: O75496 Related reagents
Entrez Gene: GMNN Related reagents
GO Terms: GO:0000216 M/G1 transition of mitotic cell cycle; GO:0005829 cytosol; GO:0005654 nucleoplasm; GO:0008156 negative regulation of DNA replication; GO:0016564 transcription repressor activity; GO:0032582 negative regulation of gene-specific transcription; GO:0042826 histone deacetylase binding; GO:0045786 negative regulation of cell cycle
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of GMNN antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

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