Glutathione Synthetase antibody | OTI2F2
Mouse anti Glutathione Synthetase
Mouse anti human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p, or glutathione synthase. This antibody has been extensively validated for western blotting using whole cell lysates.
Glutathione Synthetase Antibody with Control Lysate
Mouse anti human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p, epididymis secretory sperm binding protein Li 88n or glutathione synthase.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2F2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00364 | WB | 100 µl | ||||
| VMA00364KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p and epididymis secretory sperm binding protein Li 88n.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).
Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).
Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti glutathione synthetase detects a band of approximately 52 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human glutathione synthetase (NP_000169) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HeLa lysate lyophilized in RIPA buffer
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch
More Information
- UniProt
- P48637 Related reagents
- Entrez Gene
- GSS Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004363 glutathione synthase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006805 xenobiotic metabolic process
- GO:0006979 response to oxidative stress
- GO:0007399 nervous system development
- GO:0042803 protein homodimerization activity
- GO:0043295 glutathione binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Glutathione Synthetase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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