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Glutathione Synthetase antibody | OTI2F2

Mouse anti Glutathione Synthetase

Product Type
PrecisionAb Monoclonal
Clone
OTI2F2
Isotype
IgG1
Format
Purified
Specificity
Glutathione Synthetase

Product Code Applications Pack Size List Price Your Price Qty
VMA00364
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human glutathione synthetase antibody, clone OTI2F2 recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p and epididymis secretory sperm binding protein Li 88n.

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).

Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glutathione synthetase detects a band of approximately 52 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human glutathione synthetase (NP_000169) produced in HEK293T cells
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P48637
Entrez Gene
GSS
GO Terms
GO:0000287 magnesium ion binding
GO:0004363 glutathione synthase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006805 xenobiotic metabolic process
GO:0006979 response to oxidative stress
GO:0007399 nervous system development
GO:0042803 protein homodimerization activity
GO:0043295 glutathione binding

VMA00364

160322

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