Glutathione Synthetase antibody | OTI2F2

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Mouse anti Glutathione Synthetase

Mouse anti human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p, or glutathione synthase. This antibody has been extensively validated for western blotting using whole cell lysates.

Glutathione Synthetase Antibody with Control Lysate

Mouse anti human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p, epididymis secretory sperm binding protein Li 88n or glutathione synthase.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI2F2
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00364 WB 100 µl
VMA00364KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p and epididymis secretory sperm binding protein Li 88n.

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).

Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti glutathione synthetase detects a band of approximately 52 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human glutathione synthetase (NP_000169) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P48637 Related reagents
Entrez Gene
GSS Related reagents
GO Terms
GO:0000287 magnesium ion binding
GO:0004363 glutathione synthase activity
GO:0005829 cytosol
GO:0005524 ATP binding
GO:0006805 xenobiotic metabolic process
GO:0006979 response to oxidative stress
GO:0007399 nervous system development
GO:0042803 protein homodimerization activity
GO:0043295 glutathione binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Glutathione Synthetase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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