Glutathione Synthetase antibody | OTI2F2
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WB ResetMouse anti Glutathione Synthetase
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI2F2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- Glutathione Synthetase
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| Mouse anti Human glutathione synthetase antibody, clone OTI2F2 recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p and epididymis secretory sperm binding protein Li 88n. Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008). Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
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- Target Species
- Human
- Western Blotting
- Anti glutathione synthetase detects a band of approximately 52 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human glutathione synthetase (NP_000169) produced in HEK293T cells
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- P48637
- Entrez Gene
- GSS
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004363 glutathione synthase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006805 xenobiotic metabolic process
- GO:0006979 response to oxidative stress
- GO:0007399 nervous system development
- GO:0042803 protein homodimerization activity
- GO:0043295 glutathione binding
- View More GO Terms
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