Glutathione Synthetase antibody | OTI2F2
Mouse anti Glutathione Synthetase
Mouse anti human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p, or glutathione synthase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2F2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00364 | WB | 100 µl |
Mouse anti Human glutathione synthetase antibody recognizes glutathione synthetase, also known as GSH synthetase, GSS, GSH-S, epididymis secretory sperm binding protein Li 64p and epididymis secretory sperm binding protein Li 88n.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).
Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. Encoded by the GSS gene, glutathione synthetase functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in GSS are a cause of glutathione synthetase deficiency (provided by RefSeq, Jul 2008).
Mouse anti Human glutathione synthetase antibody detects a band of 52 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti glutathione synthetase detects a band of approximately 52 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human glutathione synthetase (NP_000169) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P48637 Related reagents
- Entrez Gene
- GSS Related reagents
- GO Terms
- GO:0000287 magnesium ion binding
- GO:0004363 glutathione synthase activity
- GO:0005829 cytosol
- GO:0005524 ATP binding
- GO:0006805 xenobiotic metabolic process
- GO:0006979 response to oxidative stress
- GO:0007399 nervous system development
- GO:0042803 protein homodimerization activity
- GO:0043295 glutathione binding
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Glutathione Synthetase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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