Glucocorticoid Receptor antibody | OTI2C4

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Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of Glucocorticoid Receptor knockout HeLa and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti Glucocorticoid Receptor antibody (**VMA00218**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 209 second and (B) 30 second exposure.

Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) gallery image 2 — Normalized signal intensity of Glucocorticoid Receptor from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Anti Glucocorticoid Receptor Antibody, clone OTI2C4 (PrecisionAb Monoclonal Antibody) gallery image 3 — Western blot analysis of whole cell lysates probed with Mouse anti Human glucocorticoid receptor antibody, clone OTI2C4 (**VMA00218**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 17 second exposure. Arrow points to glucocorticoid receptor (molecular weight 94 kDa).

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Mouse anti Glucocorticoid Receptor

Product Type: PrecisionAb Monoclonal
Clone: OTI2C4
Isotype: IgG2b
Format: Purified
Specificity: Glucocorticoid Receptor

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VMA00218 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human glucocorticoid receptor antibody, clone OTI2C4 recognizes the glucocorticoid receptor, also known as glucocorticoid nuclear receptor variant 1.

Encoded by the NR3C1 gene, the glucocorticoid receptor can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in NR3C1 are associated with generalized glucocorticoid resistance. Alternative splicing of NR3C1 results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (Lu & Cidlowski 2005).

Mouse anti Human glucocorticoid receptor antibody, clone OTI2C4 detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti glucocorticoid receptor detects a band of approximately 94 kDa in HeLa cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 1-265 of human glucocorticoid receptor produced in E. coli
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P04150
Entrez Gene: NR3C1
GO Terms: GO:0005515 protein binding; GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0004883 glucocorticoid receptor activity; GO:0005496 steroid binding; GO:0005654 nucleoplasm; GO:0005759 mitochondrial matrix; GO:0006366 transcription from RNA polymerase II promoter; GO:0008270 zinc ion binding; GO:0016568 chromatin modification; View More GO Terms; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0043565 sequence-specific DNA binding; GO:0051789 response to protein stimulus; GO:0071109 superior temporal gyrus development