Glucocorticoid Receptor antibody | OTI2C4

Mouse anti Glucocorticoid Receptor

Product Type
PrecisionAb Monoclonal
Clone
OTI2C4
Isotype
IgG2b
Format
Purified
Specificity
Glucocorticoid Receptor

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VMA00218
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Mouse anti Human glucocorticoid receptor antibody, clone OTI2C4 recognizes the glucocorticoid receptor, also known as glucocorticoid nuclear receptor variant 1.

Encoded by the NR3C1 gene, the glucocorticoid receptor can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in NR3C1 are associated with generalized glucocorticoid resistance. Alternative splicing of NR3C1 results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (Lu & Cidlowski 2005).

Mouse anti Human glucocorticoid receptor antibody, clone OTI2C4 detects a band of 94 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti glucocorticoid receptor detects a band of approximately 94 kDa in HeLa cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 1-265 of human glucocorticoid receptor produced in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P04150
Entrez Gene
NR3C1
GO Terms
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0004883 glucocorticoid receptor activity
GO:0005496 steroid binding
GO:0005654 nucleoplasm
GO:0005759 mitochondrial matrix
GO:0006366 transcription from RNA polymerase II promoter
GO:0008270 zinc ion binding
GO:0016568 chromatin modification
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0043565 sequence-specific DNA binding
GO:0051789 response to protein stimulus
GO:0071109 superior temporal gyrus development