GEP100 antibody | AbD39152eg

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Human anti GEP100

Product Type
PrecisionAb Monoclonal
Clone
AbD39152eg
Isotype
HuCAL Fab bivalent
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00897
Datasheet
WB
SDS
0.1 mg loader

Human anti GEP100 antibody recognizes IQ motif and SEC7 domain-containing protein 1, also known as ARFGEP100 and BRAG2.

GEP100 is a guanine nucleotide exchange factor for ARF6, a component of the RAS superfamily involved with membrane trafficking (You et al. 2019). AMAP1 is then the downstream effector of activated ARF6 (Kinoshita et al. 2013). GEP100 appears to control oncogenic processes in response to stimulation of EGF (Hu et al. 2013), also interacting specifically with EGF R (Hu et al. 2012). For example, in breast cancer, activation of ARF6 through GEP100 leads to invasion and metastasis through the EGF R pathway. GEP100 overexpression alongside ARF6 can also cause noninvasive MCF7 cells to become invasive (Sabe et al. 2009).

Product Details

Target Species
Human
Western Blotting
Human anti GEP100 antibody detects a band of approximately 112 kDa in MCF7, HepG2, K562, A431 cell lysates
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via an inactive variant of the bacterial alkaline phosphatase (BAP) fusion protein. The antibody is tagged with DYKDDDDK and His (HHHHHH) tags. This antibody is supplied as a liquid.
Preparation
Prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
GEP100 peptide coupled to BSA and TRF and biotinylated peptide (RLRSSMSENRMSRRI-C and Bio-ERLRSSMSENRMSRRIV)
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q6DN90
Entrez Gene
IQSEC1
GO Terms
GO:0005086 ARF guanyl-nucleotide exchange factor activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0032012 regulation of ARF protein signal transduction
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of GEP100 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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