GAPDH antibody | AbD22549
hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.
Features and Benefits
Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin
Storage Information
- Storage
- 1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P04406
- Entrez Gene
- GAPDH
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. - Regulatory
- For research purposes only
Applications of GAPDH antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1,000 | 1/10,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg | ||
| Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) | STAR117D800GA | F IF WB | 0.1 mg |
|
|
| Goat ANTI-MOUSE IgG StarBright™ Blue 700 | 12004158 | WB | 400 µl |
|
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| Goat ANTI-RABBIT IgG StarBright™ Blue 700 | 12004161 | WB | 400 µl |
|
|
| ANTI-TUBULIN hFAB™ Rhodamine Antibody | 12004165 | WB | 200 µl |
|
|
| ANTI-TUBULIN hFAB™ Rhodamine Antibody | 12004166 | WB | 40 µl |
|
|
| ANTI-ACTIN hFAB™ Rhodamine Antibody | 12004163 | WB | 200 µl |
|
|
| ANTI-ACTIN hFAB™ Rhodamine Antibody | 12004164 | WB | 40 µl |
|
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
