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GAPDH antibody | AbD22549

ANTI-GAPDH hFAB™ Rhodamine Antibody

Product Type
Monoclonal Antibody
Clone
AbD22549
Isotype
HuCAL Fab monovalent
Specificity
GAPDH

Product Code Applications Pack Size List Price Your Price Qty
12004168
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 40 µl loader
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loader
12004167
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 200 µl loader
List Price Your Price
loader

hFAB Rhodamine Housekeeping Protein Fluorescent Primary Antibodies allow one-step detection of common housekeeping proteins like actin, tubulin, and GAPDH in your samples. These anti-actin, anti-tubulin, and anti-GAPDH antibodies are directly labeled with a rhodamine derivative (Exmax/Emmax = 530 nm/580 nm). The hFAB Rhodamine Antibodies were generated with Human Combinatorial Antibody Library (HuCAL®) Technology, providing exceptional specificity.

These housekeeping protein antibodies can be used for protein normalization when the protein loading amounts are in the linear dynamic range. hFAB Rhodamine Antibodies can be imaged with the ChemiDoc™ MP Imaging System, and can be used in multiplex experiments with StarBright™ Blue 700 Secondary Antibodies as well as other traditional fluorescent antibodies, permitting detection of multiple target proteins and the housekeeping protein in a single blot.

Features and Benefits

Simple — No requirement for a secondary antibody; one-step detection of housekeeping proteins using highly cross-adsorbed antibodies for high specificity and sensitivity
Versatile — Compatible with use of a wide range of other fluorophores including StarBright Blue 700 and DyLight 800 Fluorophores for multiplex western blotting
Unique — HuCAL Technology eliminates cross-reactivity with primary/secondary antibodies from other species
Diverse — Antibodies have been tested to detect human or mouse housekeeping proteins

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A monovalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. and purified using NiNTA affinity chromatography. This antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain and conjugated to rhodamine - lyophilized
Reconstitution
Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at maximum speed on a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube.
Buffer Solution
12004168: Phosphate buffered saline.
12004167: Phosphate buffered saline
Preservative Stabilisers
12004168: >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% bovine serum albumin
12004167: >80% Sucrose
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% bovine serum albumin.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Rhodamine 530 580
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

1 year at -20°C lyophilized; 6 months at +4°C after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf.
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004163.pdf.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat ANTI-MOUSE IgG StarBright™ Blue 700 12004158 WB 400 µl loader
List Price Your Price
loader
Description Goat ANTI-MOUSE IgG StarBright™ Blue 700
Goat ANTI-RABBIT IgG StarBright™ Blue 700 12004161 WB 400 µl loader
List Price Your Price
loader
Description Goat ANTI-RABBIT IgG StarBright™ Blue 700
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004163 WB 200 µl loader
List Price Your Price
loader
Description ANTI-ACTIN hFAB™ Rhodamine Antibody
ANTI-ACTIN hFAB™ Rhodamine Antibody 12004164 WB 40 µl loader
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loader
Description ANTI-ACTIN hFAB™ Rhodamine Antibody
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004165 WB 200 µl loader
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loader
Description ANTI-TUBULIN hFAB™ Rhodamine Antibody
ANTI-TUBULIN hFAB™ Rhodamine Antibody 12004166 WB 40 µl loader
List Price Your Price
loader
Description ANTI-TUBULIN hFAB™ Rhodamine Antibody
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)

References for GAPDH antibody

  1. Meissner, M.E. et al. (2022) Differential Activity of APOBEC3F, APOBEC3G, and APOBEC3H in the Restriction of HIV-2.
    J Mol Biol. 434 (2): 167355.
  2. Kasamatsu, H. et al. (2023) A cysteine proteinase inhibitor ALLN alleviates bleomycin-induced skin and lung fibrosis.
    Arthritis Res Ther. 25 (1): 156.
  3. Kurumiya, E. et al. (2024) Eliglustat exerts anti-fibrotic effects by activating SREBP2 in TGF-β1-treated myofibroblasts derived from patients with idiopathic pulmonary fibrosis.
    Eur J Pharmacol. 966: 176366.

UniProt
P04406
Entrez Gene
GAPDH
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis
View more products with GAPDH specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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