Focal Adhesion Kinase antibody | OTI4A8

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Mouse anti Focal Adhesion Kinase

Focal adhesion kinase antibody recognizes focal adhesion kinase 1, also known as FADK 1, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, focal adhesion kinase isoform FAK-Del33, or focal adhesion kinase-related nonkinase.

Focal Adhesion Kinase Antibody with Control Lysate

Focal adhesion kinase antibody recognizes focal adhesion kinase 1, also known as FADK 1, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, focal adhesion kinase isoform FAK-Del33, or focal adhesion kinase-related nonkinase.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI4A8
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00167 WB 100 µl
VMA00167KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human focal adhesion kinase 1 antibody recognizes focal adhesion kinase 1, also known as FADK 1, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, focal adhesion kinase 1, focal adhesion kinase isoform FAK-Del33, focal adhesion kinase-related nonkinase, protein phosphatase 1 regulatory subunit 71 and protein phosphatase 1, regulatory subunit 71.

Encoded by the PTK2 gene, focal adhesion kinase 1 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of focal adhesion kinase 1 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for PTK2, but the full-length natures of only three of them have been determined (provided by RefSeq, Dec 2010).

Mouse anti Human focal adhesion kinase 1 antibody detects a band of 125 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti focal adhesion kinase detects a band of approximately 125 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant protein of human focal adhesion kinase (NP_722560) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A549 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q05397 Related reagents
Entrez Gene
PTK2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0004871 signal transducer activity
GO:0005524 ATP binding
GO:0005856 cytoskeleton
GO:0005925 focal adhesion
GO:0006468 protein phosphorylation
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007172 signal complex assembly
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042169 SH2 domain binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Focal Adhesion Kinase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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