Flii antibody | AB01/1C12
Mouse anti FLII antibody recognizes protein flightless-1 homolog, also known as FLIL.
FLII is a member of the actin-remodelling gelsolin protein superfamily. The protein plays a role in cell migration, co-localizing with small GTPases in actin-rich structures at the leading edge of motile cells, and interacting directly with Ras (Marei et al. 2016). FLII is involved with skin development, formation of tight junctions, and skin recovery after injury. Overexpression of FLII appears to contribute to inflammatory skin conditions, such as psoriasiform dermatitis and atopic dermatitis, and inhibition of FLII has been suggested as a potential therapy (Kopecki et al. 2018). FLII interacts with a variety of proteins involved with cell signaling, and appears to regulate activity of multiple transcription factors. It acts as a tumor suppressor in prostate cancer (Wang et al. 2016), promotes progression of breast cancer by impeding selective autophagy (He et al. 2018), and is implicated in colorectal cancer, hepatocellular carcinoma, and lung cancer (Wang et al. 2017).
FLII is a member of the actin-remodelling gelsolin protein superfamily. The protein plays a role in cell migration, co-localizing with small GTPases in actin-rich structures at the leading edge of motile cells, and interacting directly with Ras (Marei et al. 2016). FLII is involved with skin development, formation of tight junctions, and skin recovery after injury. Overexpression of FLII appears to contribute to inflammatory skin conditions, such as psoriasiform dermatitis and atopic dermatitis, and inhibition of FLII has been suggested as a potential therapy (Kopecki et al. 2018). FLII interacts with a variety of proteins involved with cell signaling, and appears to regulate activity of multiple transcription factors. It acts as a tumor suppressor in prostate cancer (Wang et al. 2016), promotes progression of breast cancer by impeding selective autophagy (He et al. 2018), and is implicated in colorectal cancer, hepatocellular carcinoma, and lung cancer (Wang et al. 2017).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti FLII antibody detects a band of approximately 148 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 801-1269 of human FLII
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q13045
- Entrez Gene
- FLII
- GO Terms
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0005634 nucleus
- GO:0005813 centrosome
- GO:0006350 transcription
- GO:0007275 multicellular organismal development
- GO:0045449 regulation of transcription
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of Flii antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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