FHL2 antibody | AB04-4H8
Mouse anti FHL2 antibody recognizes four and a half LIM domains protein 2, also known as DRAL and SLIM3.
FLH2 is the best-studied of the FHL proteins (Sun et al. 2018) and has the ability to modulate signalling pathways involved with diverse cellular functions. Based on its structure FLH2 seems to primarily function as an adaptor or scaffold protein (Cai et al. 2018), and fulfils its diverse range of functions owing to differential use of its LIM domains. These allow FHL2 to interact with a broad range of unrelated molecules (Kleiber et al. 2007). FHL2 is strongly related to cancer progression. Expression patterns of FHL2 are very different between different types of cancer, and FLH2 can act as either a tumor suppressor or an oncogene depending on the tissue type (Cao et al. 2015).
FLH2 is the best-studied of the FHL proteins (Sun et al. 2018) and has the ability to modulate signalling pathways involved with diverse cellular functions. Based on its structure FLH2 seems to primarily function as an adaptor or scaffold protein (Cai et al. 2018), and fulfils its diverse range of functions owing to differential use of its LIM domains. These allow FHL2 to interact with a broad range of unrelated molecules (Kleiber et al. 2007). FHL2 is strongly related to cancer progression. Expression patterns of FHL2 are very different between different types of cancer, and FLH2 can act as either a tumor suppressor or an oncogene depending on the tissue type (Cao et al. 2015).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse FHL2 antibody detects a band of approximately 33 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-279 of human FHL2
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q14192
- Entrez Gene
- FHL2
- GO Terms
- GO:0003713 transcription coactivator activity
- GO:0005634 nucleus
- GO:0005925 focal adhesion
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0009725 response to hormone stimulus
- GO:0015629 actin cytoskeleton
- GO:0030521 androgen receptor signaling pathway
- GO:0044255 cellular lipid metabolic process
- GO:0045893 positive regulation of transcription, DNA-dependent
- GO:0050681 androgen receptor binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of FHL2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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