FHL2 antibody | AB04-4H8

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Anti FHL2 Antibody, clone AB04-4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti FHL2 Antibody, clone AB04-4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti FHL2 Antibody, clone AB04-4H8 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with FHL2 antibody (1/1,000, **VMA00791**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to FHL2 (molecular weight 33 kDa)

Anti FHL2 Antibody, clone AB04-4H8 (PrecisionAb Monoclonal Antibody) gallery image 2 — Immunoprecipitation of FHL2 from HeLa whole cell lysate. The Input lane is 10% input; the FHL2-IP lane is the immunoprecipitation with the FHL2 antibody (2.5 µg, **VMA00791**); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using FHL2 antibody (1/1000, **VMA00791**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, 1705047) and visualized on the ChemiDoc MP. Arrow points to FHL2.

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WB IP

Mouse anti FHL2

Product Type: PrecisionAb Monoclonal
Clone: AB04-4H8
Isotype: IgG1
Format: Purified
Specificity: FHL2

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VMA00791 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Mouse anti FHL2 antibody recognizes four and a half LIM domains protein 2, also known as DRAL and SLIM3.

FLH2 is the best-studied of the FHL proteins (Sun et al. 2018) and has the ability to modulate signalling pathways involved with diverse cellular functions. Based on its structure FLH2 seems to primarily function as an adaptor or scaffold protein (Cai et al. 2018), and fulfils its diverse range of functions owing to differential use of its LIM domains. These allow FHL2 to interact with a broad range of unrelated molecules (Kleiber et al. 2007). FHL2 is strongly related to cancer progression. Expression patterns of FHL2 are very different between different types of cancer, and FLH2 can act as either a tumor suppressor or an oncogene depending on the tissue type (Cao et al. 2015).

Target Species: Human
Western Blotting: Mouse FHL2 antibody detects a band of approximately 33 kDa in HeLa cell lysates
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: E. coli-derived recombinant protein of amino acids 1-279 of human FHL2
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: Q14192
Entrez Gene: FHL2
GO Terms: GO:0003713 transcription coactivator activity; GO:0005634 nucleus; GO:0005925 focal adhesion; GO:0008270 zinc ion binding; GO:0042802 identical protein binding; GO:0009725 response to hormone stimulus; GO:0015629 actin cytoskeleton; GO:0030521 androgen receptor signaling pathway; GO:0044255 cellular lipid metabolic process; View More GO Terms; GO:0045893 positive regulation of transcription, DNA-dependent; GO:0050681 androgen receptor binding