FHL2 antibody | AB04-4H8

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Mouse anti FHL2

Product Type
PrecisionAb Monoclonal
Clone
AB04-4H8
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00791
Datasheet
IP WB
SDS
100 µl loader

Mouse anti FHL2 antibody recognizes four and a half LIM domains protein 2, also known as DRAL and SLIM3.

FLH2 is the best-studied of the FHL proteins (Sun et al. 2018) and has the ability to modulate signalling pathways involved with diverse cellular functions. Based on its structure FLH2 seems to primarily function as an adaptor or scaffold protein (Cai et al. 2018), and fulfils its diverse range of functions owing to differential use of its LIM domains. These allow FHL2 to interact with a broad range of unrelated molecules (Kleiber et al. 2007). FHL2 is strongly related to cancer progression. Expression patterns of FHL2 are very different between different types of cancer, and FLH2 can act as either a tumor suppressor or an oncogene depending on the tissue type (Cao et al. 2015).

Product Details

Target Species
Human
Western Blotting
Mouse FHL2 antibody detects a band of approximately 33 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-279 of human FHL2
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
Q14192
Entrez Gene
FHL2
GO Terms
GO:0003713 transcription coactivator activity
GO:0005634 nucleus
GO:0005925 focal adhesion
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0009725 response to hormone stimulus
GO:0015629 actin cytoskeleton
GO:0030521 androgen receptor signaling pathway
GO:0044255 cellular lipid metabolic process
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0050681 androgen receptor binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of FHL2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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