Estrogen Receptor Alpha antibody

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Mouse anti Estrogen Receptor Alpha

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00231
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human estrogen receptor alpha antibody recognizes estrogen receptor alpha, also known as ER-alpha, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2 and nuclear receptor subfamily 3 group A member 1.

Encoded by the ESR1 gene, estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters (provided by RefSeq, Jul 2008).

Mouse anti Human estrogen receptor alpha antibody detects a band of 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti estrogen receptor alpha detects a band of approximately 66 kDa in C6 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human estrogen receptor alpha expressed in E. coli

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P03372 Related reagents
Entrez Gene
ESR1 Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005496 steroid binding
GO:0005737 cytoplasm
GO:0005654 nucleoplasm
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0016585 chromatin remodeling complex
GO:0019899 enzyme binding
GO:0030235 nitric-oxide synthase regulator activity
GO:0030284 estrogen receptor activity
GO:0032355 response to estradiol stimulus
GO:0034056 estrogen response element binding
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0048386 positive regulation of retinoic acid receptor signaling pathway
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Estrogen Receptor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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