Estrogen Receptor Alpha antibody

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Anti Estrogen Receptor Alpha Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human estrogen receptor alpha antibody (**VMA00231**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 3 second exposure. Arrow points to estrogen receptor alpha (molecular weight 66 kDa).

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Mouse anti Estrogen Receptor Alpha

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Estrogen Receptor Alpha

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Product Code Applications Pack Size List Price Your Price Qty

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VMA00231 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human estrogen receptor alpha antibody recognizes estrogen receptor alpha, also known as ER-alpha, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2 and nuclear receptor subfamily 3 group A member 1.

Encoded by the ESR1 gene, estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters (provided by RefSeq, Jul 2008).

Mouse anti Human estrogen receptor alpha antibody detects a band of 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti estrogen receptor alpha detects a band of approximately 66 kDa in C6 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant fragment of human estrogen receptor alpha expressed in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P03372
Entrez Gene: ESR1
GO Terms: GO:0003700 sequence-specific DNA binding transcription factor activity; GO:0005496 steroid binding; GO:0005737 cytoplasm; GO:0005654 nucleoplasm; GO:0008013 beta-catenin binding; GO:0008270 zinc ion binding; GO:0016585 chromatin remodeling complex; GO:0019899 enzyme binding; GO:0030235 nitric-oxide synthase regulator activity; View More GO Terms; GO:0030284 estrogen receptor activity; GO:0032355 response to estradiol stimulus; GO:0034056 estrogen response element binding; GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor; GO:0048386 positive regulation of retinoic acid receptor signaling pathway