Estrogen Receptor Alpha antibody
Mouse anti Human estrogen receptor alpha antibody recognizes estrogen receptor alpha, also known as ER-alpha, estradiol receptor, estrogen nuclear receptor alpha, estrogen receptor, estrogen receptor alpha E1-E2-1-2, estrogen receptor alpha E1-N2-E2-1-2 and nuclear receptor subfamily 3 group A member 1.
Encoded by the ESR1 gene, estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters (provided by RefSeq, Jul 2008).
Mouse anti Human estrogen receptor alpha antibody detects a band of 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ESR1 gene, estrogen receptor alpha is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters (provided by RefSeq, Jul 2008).
Mouse anti Human estrogen receptor alpha antibody detects a band of 66 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti estrogen receptor alpha detects a band of approximately 66 kDa in C6 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified recombinant fragment of human estrogen receptor alpha expressed in E. coli
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P03372
- Entrez Gene
- ESR1
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0005496 steroid binding
- GO:0005737 cytoplasm
- GO:0005654 nucleoplasm
- GO:0008013 beta-catenin binding
- GO:0008270 zinc ion binding
- GO:0016585 chromatin remodeling complex
- GO:0019899 enzyme binding
- GO:0030235 nitric-oxide synthase regulator activity
- GO:0030284 estrogen receptor activity
- GO:0032355 response to estradiol stimulus
- GO:0034056 estrogen response element binding
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- GO:0048386 positive regulation of retinoic acid receptor signaling pathway
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Estrogen Receptor Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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