ErbB2 antibody | OTI4F10

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Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ErbB2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 12 second exposure. Arrow points to ErbB2 (molecular weight 185 kDa)

Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of ErbB2 knockout HeLa and wild type HeLa (WT) whole cell lysates probed with Mouse anti ErbB2 antibody (**VMA00147**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and visualized on the ChemiDoc MP with a 60 second exposure. Tubulin was detected with hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a 30 second exposure.

Anti ErbB2 Antibody, clone OTI4F10 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of ErbB2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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Mouse anti ErbB2

Product Type: PrecisionAb Monoclonal
Clone: OTI4F10
Isotype: IgG2a
Format: Purified
Specificity: ErbB2

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VMA00147 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human ErbB2 antibody recognizes ErbB2, also known as c-erb B2/neu protein, herstatin, metastatic lymph node gene 19 protein, neuro/glioblastoma derived oncogene homolog, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, proto-oncogene Neu, proto-oncogene c-ErbB-2, receptor tyrosine-protein kinase erbB-2, tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2, v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 and neuro/glioblastoma derived oncogene homolog.

Encoded by the ERBB2 gene, ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of ERBB2 has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized (provided by RefSeq, Jul 2008).

Mouse anti Human ErbB2 antibody detects a band of 185 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti ErbB2 detects a band of approximately 185 kDa in HepG2 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein corresponding to amino acids 676-1255 of human ErbB2 (NP_004439) produced in HEK293T cells
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

Synonyms: HER2; MLN19; Neu; Ngl
UniProt: P04626
Entrez Gene: ERBB2
GO Terms: GO:0003677 DNA binding; GO:0016021 integral to membrane; GO:0004716 receptor signaling protein tyrosine kinase activity; GO:0005006 epidermal growth factor receptor activity; GO:0043125 ErbB-3 class receptor binding; GO:0005524 ATP binding; GO:0005634 nucleus; GO:0006350 transcription; GO:0007507 heart development; View More GO Terms; GO:0008022 protein C-terminus binding; GO:0008283 cell proliferation; GO:0042802 identical protein binding; GO:0014065 phosphatidylinositol 3-kinase cascade; GO:0019903 protein phosphatase binding; GO:0048015 phosphatidylinositol-mediated signaling; GO:0042060 wound healing; GO:0043235 receptor complex; GO:0043406 positive regulation of MAP kinase activity; GO:0045449 regulation of transcription; GO:0045765 regulation of angiogenesis; GO:0045785 positive regulation of cell adhesion; GO:0046777 protein autophosphorylation; GO:0046982 protein heterodimerization activity; GO:0048471 perinuclear region of cytoplasm; GO:0050679 positive regulation of epithelial cell proliferation