EIF3H antibody | AB04/3F5

100% Secure


Mouse anti EIF3H

Product Type
PrecisionAb Monoclonal
Clone
AB04/3F5
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00935
Datasheet
IP WB
SDS
100 µl loader

Mouse anti EIF3H antibody recognizes eukaryotic translation initiation factor 3 subunit H, also known as EIF3S3.

EIF3H is a subunit of the mammalian EIF3 complex, and contributes to mRNA recruitment and ribosomal complex disassembly. EIF3H was found to be involved with regulation of cell growth and viability, which led to the suggestion that it could provide a growth advantage to cancer cells (Savinainen et al. 2006). Accordingly, it has been identified as a known driver of cell proliferation and survival in cancer, overexpressed in prostate, breast, and liver cancer, and has been associated with poor prognosis (Cappuzzo et al. 2009). EIF3H knockdown can lead to apoptosis of colorectal cancer cells, leading to its suggestion as a potential biomarker (Yu et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti EIF3H detects a band of approximately 40 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-352 of human EIF3H
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
O15372
Entrez Gene
EIF3H
GO Terms
GO:0005515 protein binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0005852 eukaryotic translation initiation factor 3 complex
GO:0006446 regulation of translational initiation
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of EIF3H antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

Fluorescent Spectraviewer

Watch the Tool Tutorial Video ▸

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra