EIF2S1 antibody | OTI3H4
Filter by Application:
WB ResetMouse anti EIF2S1
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI3H4
- Isotype
- IgG1
- Format
- Purified
- Specificity
- EIF2S1
Antibody Quality and Performance Guarantee
| Mouse anti Human EIF2S1 antibody, clone OTI3H4 recognizes EIF2S1, also known as eIF-2-alpha, eukaryotic translation initiation factor 2 subunit 1 and eukaryotic translation initiation factor 2 subunit alpha. The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36 kDa EIF2-alpha subunit (EIF2S1), the 38 kDa EIF2-beta subunit (EIF2S2), and the 52 kDa EIF2-gamma subunit (EIF2S3). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. 1987). Mouse anti Human EIF2S1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti EIF2S1 detects a band of approximately 36 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human EIF2S1 (NP_004085) produced in HEK293T cells
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
Further Reading
-
Ernst H et al. (1987) Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA.
J Biol Chem. 262 (3): 1206-12.
- UniProt
- P05198
- Entrez Gene
- EIF2S1
- GO Terms
- GO:0005515 protein binding
- GO:0003743 translation initiation factor activity
- GO:0005829 cytosol
- GO:0005844 polysome
- GO:0005850 eukaryotic translation initiation factor 2 complex
- GO:0043022 ribosome binding
VMA00134
240615
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up