EIF2S1 antibody | OTI3H4

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Mouse anti EIF2S1

Product Type
PrecisionAb Monoclonal
Clone
OTI3H4
Isotype
IgG1
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00134
Datasheet
WB
MSDS
100 µl loader

Mouse anti Human EIF2S1 antibody recognizes EIF2S1, also known as eIF-2-alpha, eukaryotic translation initiation factor 2 subunit 1 and eukaryotic translation initiation factor 2 subunit alpha.

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36 kDa EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2), and the 52-kD EIF2-gamma subunit (EIF2S3). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al. 1987).

Mouse anti Human EIF2S1 antibody detects a band of 36 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti EIF2S1 detects a band of approximately 36 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human EIF2S1 (NP_004085) produced in HEK293T cells

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Guarantee
As supplied, 12 months from date of despatch.

More Information

UniProt
P05198 Related reagents
Entrez Gene
EIF2S1 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003743 translation initiation factor activity
GO:0005829 cytosol
GO:0005844 polysome
GO:0005850 eukaryotic translation initiation factor 2 complex
GO:0043022 ribosome binding
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of EIF2S1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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