EGF R antibody | C225
0.5 μg of Human Anti-EGF R Antibody, MCA6102 (cetuximab biosimilar, research grade) and cetuximab (therapeutic reference product) were denatured and analyzed under reducing (R) and non-reducing (NR) conditions. Bands were visualized using Coomassie staining. Full IgG (H2L2), heavy (H) and light chains (L) are indicated.
Size exclusion chromatography (SEC) of Human Anti-EGF R Antibody, MCA6102 (cetuximab biosimilar, research grade) and cetuximab (therapeutic reference product) was performed on an analytical HPLC instrument. Shown are monomer peaks (H2L2), calculated relative monomer portion and aggregates. Column: MAbPac SEC-1, 5 μm; dimension: 4.0 x 150 mm; mobile phase: 50 mM sodium phosphate, pH 6.8, 300 mM NaCl, 200 mM Arginine; flow rate: 200 μl/min; inj. volume [μl]: 3; temp.: 25 °C; detection: 280 nm; sample: mAb (1 mg/ml).
A microtiter plate was coated overnight with Human Anti-Cetuximab Antibody (clone AbD19834, HCA220) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of cetuximab (therapeutic reference product) or Human Anti-EGF R Antibody, MCA6102 (cetuximab biosimilar, research grade). Detection was performed using HRP conjugated Human Anti-Cetuximab Antibody (clone AbD19830_hIgG1, HCA221P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti EGF Receptor (Cetuximab Biosimilar)
Anti-EGF R Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug cetuximab (Erbitux) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.
- Product Type
- Monoclonal Antibody
- Clone
- C225
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA6102 | E | 0.2 mg |
|
Human Anti-EGF R Antibody, clone C225 is a research grade biosimilar of the monoclonal antibody drug cetuximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic antibody cetuximab.
Human Anti-EGF R Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-cetuximab antibodies, for example HCA220 and HCA221P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-cetuximab antibody in IgG1 format as reference standard, for example HCA221.
Cetuximab (Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-cetuximab antibody products
Human Anti-EGF R Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-cetuximab antibodies, for example HCA220 and HCA221P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-cetuximab antibody in IgG1 format as reference standard, for example HCA221.
Cetuximab (Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.
This product is NOT FOR THERAPEUTIC USE.
View a summary of supporting anti-cetuximab antibody products
Product Details
- Target Species
- Human
- Product Form
- Human IgG1 (kappa) antibody - liquid
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Proclin 300
- Immunogen
- Human EGF R
- Purity
- >98% by SDS PAGE
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00533
- Entrez Gene
- EGFR
- Licensed Use
- For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
- Regulatory
- For research purposes only
Applications of EGF R antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
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