EGF R antibody | C225

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Human anti EGF Receptor (Cetuximab Biosimilar)

Anti-EGF R Antibody is a non-therapeutic biosimilar of the monoclonal antibody drug cetuximab (Erbitux) for research use. It can be used in bioanalytical assays and for studying biological pathways affected by the drug.

Product Type
Monoclonal Antibody
Clone
C225
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6102 E 0.2 mg
Human Anti-EGF R Antibody, clone C225 is a research grade biosimilar of the monoclonal antibody drug cetuximab. It is a recombinant human IgG1 kappa antibody with variable regions from the therapeutic antibody cetuximab.

Human Anti-EGF R Antibody is for research use only. It is suitable as a reference standard in a pharmacokinetic (PK) bridging ELISA with anti-cetuximab antibodies, for example HCA220 and HCA221P. It can also be used as capture and detection reagent in an anti-drug antibody (ADA) bridging ELISA, with an anti-cetuximab antibody in IgG1 format as reference standard, for example HCA221.

Cetuximab (Erbitux) is a chimeric monoclonal antibody drug (IgG1/kappa) approved for treatment of KRAS wild-type metastatic colorectal cancers and squamous cell carcinoma of the head and neck. This therapeutic antibody is directed against EGFR and inhibits tumor cell proliferation by blocking the interaction of epidermal growth factor (EGF) with its receptor.

This product is NOT FOR THERAPEUTIC USE.

View a summary of supporting anti-cetuximab antibody products

Product Details

Target Species
Human
Product Form
Human IgG1 (kappa) antibody - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Proclin 300
Immunogen
Human EGF R
Purity
>98% by SDS PAGE
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

UniProt
P00533 Related reagents
Entrez Gene
EGFR Related reagents
Licensed Use
For in vitro research purposes only. Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Regulatory
For research purposes only

Applications of EGF R antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ