Drebrin-Like Protein antibody | OTI7B7

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Mouse anti Drebrin-Like Protein

Drebrin-like protein antibody recognizes drebrin-like protein, also known as HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, cervical SH3P7, cervical mucin-associated protein, drebrin-F, or drebrin-like protein.

Drebrin-Like Protein Antibody with Control Lysate

Drebrin-like protein antibody recognizes drebrin-like protein, also known as HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, cervical SH3P7, cervical mucin-associated protein, drebrin-F, or drebrin-like protein.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI7B7
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00282 WB 100 µl
VMA00282KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human drebrin-like protein antibody recognizes debrin-like protein, also known as HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, cervical SH3P7, cervical mucin-associated protein, drebrin-F, drebrin-like protein and src homology 3 domain-containing protein HIP-55.

Mouse anti Human debrin-like protein antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti drebrin-like protein detects a band of approximately 55 kDa in HeLa cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human drebrin-like protein (NP_054782) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q9UJU6 Related reagents
Entrez Gene
DBNL Related reagents
GO Terms
GO:0003779 actin binding
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005938 cell cortex
GO:0006897 endocytosis
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007257 activation of JUN kinase activity
GO:0008047 enzyme activator activity
GO:0042802 identical protein binding
GO:0016601 Rac protein signal transduction
GO:0030027 lamellipodium
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Drebrin-Like Protein antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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