DLD antibody | OTI6D5

Mouse anti DLD

Product Type
PrecisionAb Monoclonal
Clone
OTI6D5
Isotype
IgG1
Format
Purified
Specificity
DLD

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VMA00141
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WB 100 µl loader
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Mouse anti Human DLD antibody, clone OTI6D5 recognizes dihydrolipoyl dehydrogenase (DLD), also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, diaphorase, mitochondrial, glycine cleavage system L protein, glycine cleavage system protein L, lipoamide dehydrogenase, lipoamide reductase and lipoyl dehydrogenase.

Dihydrolipoamide dehydrogenase is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in DLD have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency (provided by RefSeq, Jul 2008).

Mouse anti Human DLD antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti DLD detects a band of approximately 54 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human DLD (NP_000099) produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P09622
Entrez Gene
DLD
GO Terms
GO:0004148 dihydrolipoyl dehydrogenase activity
GO:0005759 mitochondrial matrix
GO:0006099 tricarboxylic acid cycle
GO:0045454 cell redox homeostasis
GO:0006554 lysine catabolic process
GO:0009083 branched chain family amino acid catabolic process
GO:0010510 regulation of acetyl-CoA biosynthetic process from pyruvate
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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