DLD antibody | OTI6D5

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Mouse anti DLD

DLD antibody recognizes mitochondrial dihydrolipoyl dehydrogenase, also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, diaphorase, or lipoyl dehydrogenase.

DLD Antibody with Control Lysate

DLD antibody recognizes mitochondrial dihydrolipoyl dehydrogenase, also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, diaphorase, or glycine cleavage system L protein.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI6D5
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00141 WB 100 µl
VMA00141KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human DLD antibody recognizes dihydrolipoyl dehydrogenase (DLD), also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, diaphorase, mitochondrial, glycine cleavage system L protein, glycine cleavage system protein L, lipoamide dehydrogenase, lipoamide reductase and lipoyl dehydrogenase.

Dihydrolipoamide dehydrogenase is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in DLD have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency (provided by RefSeq, Jul 2008).

Mouse anti Human DLD antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti DLD detects a band of approximately 54 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human DLD (NP_000099) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P09622 Related reagents
Entrez Gene
DLD Related reagents
GO Terms
GO:0004148 dihydrolipoyl dehydrogenase activity
GO:0005759 mitochondrial matrix
GO:0006099 tricarboxylic acid cycle
GO:0045454 cell redox homeostasis
GO:0006554 lysine catabolic process
GO:0009083 branched chain family amino acid catabolic process
GO:0010510 regulation of acetyl-CoA biosynthetic process from pyruvate
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of DLD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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