DLD antibody | OTI6D5

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Anti DLD Antibody, clone OTI6D5 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with DLD antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 4 second exposure. Arrow points to DLD (molecular weight 54 kDa)

Mouse anti DLD

DLD antibody recognizes mitochondrial dihydrolipoyl dehydrogenase, also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, diaphorase, or lipoyl dehydrogenase.

DLD Antibody with Control Lysate

DLD antibody recognizes mitochondrial dihydrolipoyl dehydrogenase, also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, diaphorase, or glycine cleavage system L protein.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI6D5
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00141	WB	100 µl
VMA00141KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human DLD antibody recognizes dihydrolipoyl dehydrogenase (DLD), also known as E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex, diaphorase, mitochondrial, glycine cleavage system L protein, glycine cleavage system protein L, lipoamide dehydrogenase, lipoamide reductase and lipoyl dehydrogenase.

Dihydrolipoamide dehydrogenase is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in DLD have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency (provided by RefSeq, Jul 2008).

Mouse anti Human DLD antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti DLD detects a band of approximately 54 kDa in K562 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human DLD (NP_000099) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg K562 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P09622 Related reagents
Entrez Gene: DLD Related reagents
GO Terms: GO:0004148 dihydrolipoyl dehydrogenase activity; GO:0005759 mitochondrial matrix; GO:0006099 tricarboxylic acid cycle; GO:0045454 cell redox homeostasis; GO:0006554 lysine catabolic process; GO:0009083 branched chain family amino acid catabolic process; GO:0010510 regulation of acetyl-CoA biosynthetic process from pyruvate
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of DLD antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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