Desmoplakin antibody

Mouse anti Desmoplakin

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
Desmoplakin
Region
aa2344-2822

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VMA00095
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IF WB 100 µl loader
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Mouse anti Human desmoplakin recognises a major protein component of the desmosome. Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is a major high molecular weight protein (312 kDa) of desmosomes which anchor intermediate filaments to desmosomal plaques.

The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 to selectively recruit plakophilins to desmosomal plaques. Desmoplakin has also been shown to function as a transglutaminase substrate in vitro, suggesting that it may participate in cell adhesion at the intra-epidermal level.

Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. Firstly, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Secondly, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. Mutations in the gene for this protein are the cause of several cardiomyopathies and keratodermas as well as some autoimmune diseases.

Mouse anti Human desmoplakin binds to an epitope between amino acids 2344-2822 of desmoplakin. This antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti desmoplakin antibody detects a band of approximately 332 kDa in HepG2 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant fusion protein fragment consisting of the carboxyl terminus of desmoplakin, fused with maltose binding protein.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for Desmoplakin antibody

  1. Sobolik-delmaire, T. et al. (2006) Carboxyl terminus of Plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly.
    J Biol Chem. 281 (25): 16962-70.

UniProt
P15924
Entrez Gene
DSP
GO Terms
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005200 structural constituent of cytoskeleton
GO:0006921 cellular component disassembly involved in apoptosis
GO:0018149 peptide cross-linking
GO:0030057 desmosome
GO:0030216 keratinocyte differentiation
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