Desmoplakin antibody

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Mouse anti Desmoplakin

Desmoplakin antibody recognizes desmoplakin, also known as 250/210 kDa paraneoplastic pemphigus antigen. This antibody has been extensively validated for western blotting using whole cell lysates.

Desmoplakin Antibody with Control Lysate

Desmoplakin antibody recognizes desmoplakin, also known as 250/210 kDa paraneoplastic pemphigus antigen. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00095 IF WB 100 µl
VMA00095KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human desmoplakin recognises a major protein component of the desmosome. Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is a major high molecular weight protein (312 kDa) of desmosomes which anchor intermediate filaments to desmosomal plaques.

The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 to selectively recruit plakophilins to desmosomal plaques. Desmoplakin has also been shown to function as a transglutaminase substrate in vitro, suggesting that it may participate in cell adhesion at the intra-epidermal level.

Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. Firstly, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Secondly, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. Mutations in the gene for this protein are the cause of several cardiomyopathies and keratodermas as well as some autoimmune diseases.

Mouse anti Human desmoplakin binds to an epitope between amino acids 2344-2822 of desmoplakin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti desmoplakin antibody detects a band of approximately 332 kDa in HepG2 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fusion protein fragment consisting of the carboxyl terminus of desmoplakin, fused with maltose binding protein.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P15924 Related reagents
Entrez Gene
DSP Related reagents
GO Terms
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005200 structural constituent of cytoskeleton
GO:0006921 cellular component disassembly involved in apoptosis
GO:0018149 peptide cross-linking
GO:0030057 desmosome
GO:0030216 keratinocyte differentiation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Desmoplakin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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