Desmoplakin antibody

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Mouse anti Desmoplakin

Product Type
PrecisionAb Monoclonal
Product Code Applications Pack Size List Price Quantity
100 µl loader

Mouse anti Human desmoplakin recognises a major protein component of the desmosome. Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is a major high molecular weight protein (312 kDa) of desmosomes which anchor intermediate filaments to desmosomal plaques.

The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 to selectively recruit plakophilins to desmosomal plaques. Desmoplakin has also been shown to function as a transglutaminase substrate in vitro, suggesting that it may participate in cell adhesion at the intra-epidermal level.

Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. Firstly, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Secondly, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. Mutations in the gene for this protein are the cause of several cardiomyopathies and keratodermas as well as some autoimmune diseases.

Mouse anti Human desmoplakin binds to an epitope between amino acids 2344-2822 of desmoplakin. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Western Blotting
Anti desmoplakin antibody detects a band of approximately 332 kDa in HepG2 cell lysates
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Recombinant fusion protein fragment consisting of the carboxyl terminus of desmoplakin, fused with maltose binding protein.

Storage Information

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0001533 cornified envelope
GO:0030674 protein binding, bridging
GO:0005200 structural constituent of cytoskeleton
GO:0006921 cellular component disassembly involved in apoptosis
GO:0018149 peptide cross-linking
GO:0030057 desmosome
GO:0030216 keratinocyte differentiation
PrecisionAb is a trademark of Bio-Rad Laboratories.
For research purposes only

Applications of Desmoplakin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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