Desmoplakin antibody

ISO 9001 Quality Assurance Manufacturing | Print

Anti Desmoplakin Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with desmoplakin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 51 second exposure. Arrow points to desmoplakin (molecular weight 332 kDa)

Filter by Application:

Mouse anti Desmoplakin

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Desmoplakin
Region: aa2344-2822

Quick Links:

Secondary Antibodies
References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00095 Datasheet Datasheet SDS SDS	IF WB	100 µl		Log in	Get Quote
	Log in

Mouse anti Human desmoplakin recognises a major protein component of the desmosome. Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is a major high molecular weight protein (312 kDa) of desmosomes which anchor intermediate filaments to desmosomal plaques.

The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 to selectively recruit plakophilins to desmosomal plaques. Desmoplakin has also been shown to function as a transglutaminase substrate in vitro, suggesting that it may participate in cell adhesion at the intra-epidermal level.

Desmosomes are the most common type of intercellular junction in vertebrate epithelial cells. They are characterized by 2 forms of interaction with other cellular structures. Firstly, they form membrane anchorage sites for intermediate-size filaments, which are seen as electron-dense plaques evident beneath the plasma membrane. Secondly, a specific membrane core domain interacts with a corresponding domain of the plasma membrane of an adjacent cell, apparently mediating intercellular adhesion in a stable way. Mutations in the gene for this protein are the cause of several cardiomyopathies and keratodermas as well as some autoimmune diseases.

Mouse anti Human desmoplakin binds to an epitope between amino acids 2344-2822 of desmoplakin. This antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti desmoplakin antibody detects a band of approximately 332 kDa in HepG2 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant fusion protein fragment consisting of the carboxyl terminus of desmoplakin, fused with maltose binding protein.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):HRP

References for Desmoplakin antibody

Sobolik-delmaire, T. et al. (2006) Carboxyl terminus of Plakophilin-1 recruits it to plasma membrane, whereas amino terminus recruits desmoplakin and promotes desmosome assembly.
J Biol Chem. 281 (25): 16962-70.

UniProt: P15924
Entrez Gene: DSP
GO Terms: GO:0001533 cornified envelope; GO:0030674 protein binding, bridging; GO:0005200 structural constituent of cytoskeleton; GO:0006921 cellular component disassembly involved in apoptosis; GO:0018149 peptide cross-linking; GO:0030057 desmosome; GO:0030216 keratinocyte differentiation