Desmoglein 2 antibody | 10D2
Mouse anti Desmoglein 2
Desmoglein 2 antibody recognizes desmoglein-2, also known as HDGC or cadherin family member 5. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- 10D2
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00092 | IF WB | 100 µl |
Mouse anti Human desmoglein 2 recognizes desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.
Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human desmoglein 2 antibody is specific for the EC1 domain of desmoglein 2 (Keim et al., 2008) and has been extensively validated for western blotting using whole cell lysates.
Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.
Mouse anti Human desmoglein 2 antibody is specific for the EC1 domain of desmoglein 2 (Keim et al., 2008) and has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti desmoglein 2 antibody detects a band of approximately 150 kDa in HeLa cell lysates
- Product Form
- Purified IgG1 - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q14126 Related reagents
- Entrez Gene
- DSG2 Related reagents
- GO Terms
- GO:0005509 calcium ion binding
- GO:0016021 integral to membrane
- GO:0006921 cellular component disassembly involved in apoptosis
- GO:0007156 homophilic cell adhesion
- GO:0030057 desmosome
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Desmoglein 2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
Product Specific References
References for Desmoglein 2 antibody
-
Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
Hybridoma (Larchmt). 27 (4): 249-58.
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