Desmoglein 2 antibody | 10D2

ISO 9001 Quality Assurance Manufacturing | Print

KO Validated

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with desmoglein 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 43 second exposure. Arrow points to desmoglein 2 (molecular weight 150 kDa)

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of desmoglein 2 CRISPR knockout HeLa (Desmoglein 2 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti desmoglein 2 antibody (**VMA00092**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166). Membrane was treated with Clarity Max (1705062) and visualized on the ChemiDoc MP with a (A) 524 second and (B) 30 second exposure.

Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of desmoglein 2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

Filter by Application:

Mouse anti Desmoglein 2

Product Type: PrecisionAb Monoclonal
Clone: 10D2
Isotype: IgG1
Format: Purified
Specificity: Desmoglein 2

Quick Links:

Secondary Antibodies
References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

Antibody Quality and Performance Guarantee

Unrivaled Technical Support

Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00092 Datasheet Datasheet SDS SDS	IF WB	100 µl		Log in	Get Quote
	Log in

Mouse anti Human desmoglein 2 recognizes desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.

Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.

Mouse anti Human desmoglein 2 antibody is specific for the EC1 domain of desmoglein 2 (Keim et al., 2008) and has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti desmoglein 2 antibody detects a band of approximately 150 kDa in HeLa cell lysates.
Product Form: Purified IgG1 - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
List Price	Your Price
	Log in
Description	Goat anti Mouse IgG (H/L):HRP

References for Desmoglein 2 antibody

Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
Hybridoma (Larchmt). 27 (4): 249-58.

UniProt: Q14126
Entrez Gene: DSG2
GO Terms: GO:0005509 calcium ion binding; GO:0016021 integral to membrane; GO:0006921 cellular component disassembly involved in apoptosis; GO:0007156 homophilic cell adhesion; GO:0030057 desmosome