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Desmoglein 2 antibody | 10D2

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Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 2 Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) thumbnail image 3
Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with desmoglein 2 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 43 second exposure. Arrow points to desmoglein 2 (molecular weight 150 kDa)
Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 2
Western blot analysis of desmoglein 2 CRISPR knockout HeLa (Desmoglein 2 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Mouse anti desmoglein 2 antibody (VMA00092) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166). Membrane was treated with Clarity Max (1705062) and visualized on the ChemiDoc MP with a (A) 524 second and (B) 30 second exposure.
Anti Desmoglein 2 Antibody, clone 10D2 (PrecisionAb Monoclonal Antibody) gallery image 3
Normalized signal intensity of desmoglein 2 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Mouse anti Desmoglein 2

Product Type
PrecisionAb Monoclonal
Clone
10D2
Isotype
IgG1
Format
Purified
Specificity
Desmoglein 2

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Product Code Applications Pack Size List Price Your Price Qty
VMA00092
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF WB 100 µl loader
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loader

Mouse anti Human desmoglein 2 recognizes desmoglein 2, a member of the desmoglein family. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, four desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily.

Desmoglein 2 is a membrane glycoprotein with four highly conserved extracellular domains (EC1 - 4) and six cytoplasmic desmoglein repeat sequences. It has a predicted molecular weight of 122 kDa and an apparent molecular weight of 150 kDa, due to post translational modification. Desmoglein 2 is a core component of the desmosome cell-cell junction between cells. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris and mutations in the gene have been associated with arrhythmogenic right ventricular dysplasia.

Mouse anti Human desmoglein 2 antibody is specific for the EC1 domain of desmoglein 2 (Keim et al., 2008) and has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti desmoglein 2 antibody detects a band of approximately 150 kDa in HeLa cell lysates.
Product Form
Purified IgG1 - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Recombinant fusion protein fragment consisting of amino acids 37-164 of desmoglein 2 which consists of the entire extracellular domain and a portion of the proregion.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

References for Desmoglein 2 antibody

  1. Keim, S.A. et al. (2008) Generation and characterization of monoclonal antibodies against the proregion of human desmoglein-2.
    Hybridoma (Larchmt). 27 (4): 249-58.

UniProt
Q14126
Entrez Gene
DSG2
GO Terms
GO:0005509 calcium ion binding
GO:0016021 integral to membrane
GO:0006921 cellular component disassembly involved in apoptosis
GO:0007156 homophilic cell adhesion
GO:0030057 desmosome

VMA00092

151106

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