Desmin antibody | AbD03744

Human anti Human Desmin

Product Type
Monoclonal Antibody
Clone
AbD03744
Isotype
HuCAL Fab bivalent
Specificity
Desmin

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HCA023
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SDS Safety Datasheet SDS
C E P * WB 0.1 mg loader
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Human anti Human desmin, clone AbD03744 recognizes desmin, a member of the class III family of intermediate filaments. Desmin constitutes part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac and smooth muscle).

Desmin is a 52 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fiber bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. Staining for desmin is useful for identification of rhabdomyosarcomas and leiomyomas.

For use in immunohistology staining we recommend the use of an anti-His tag bridging antibody, followed by visualization using the Dako Duet staining system.

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library. Expressed in E. Coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant human desmin produced in E. coli.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
6 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2 ug/ml
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 1 1/100 1/500
Western Blotting 1/200 1/2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse anti Penta Histidine Tag:HRP

RRID
AB_770095
UniProt
P17661
Entrez Gene
DES
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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