Desmin antibody | AbD03744


Desmin is a 52 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fiber bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. Staining for desmin is useful for identification of rhabdomyosarcomas and leiomyomas.
For use in immunohistology staining we recommend the use of an anti-His tag bridging antibody, followed by visualization using the Dako Duet staining system.
Product Details
- Target Species
- Human
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. Coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Recombinant human desmin produced in E. coli.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P17661
- Entrez Gene
- DES
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. His-tag is a registered trademark of EMD Biosciences.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of Desmin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 2 ug/ml | ||
Immunohistology - Frozen | 1/100 | 1/500 | |
Immunohistology - Paraffin 1 | 1/100 | 1/500 | |
Western Blotting | 1/200 | 1/2000 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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