Desmin antibody | AbD03744

100% Secure


Human anti Human Desmin

Product Type
Monoclonal Antibody
Clone
AbD03744
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA023 C E P* WB 0.1 mg
Human anti Human desmin, clone AbD03744 recognizes desmin, a member of the class III family of intermediate filaments. Desmin constitutes part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac and smooth muscle).

Desmin is a 52 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fiber bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. Staining for desmin is useful for identification of rhabdomyosarcomas and leiomyomas.

For use in immunohistology staining we recommend the use of an anti-His tag bridging antibody, followed by visualization using the Dako Duet staining system.

Product Details

Target Species
Human
Product Form
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. Coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant human desmin produced in E. coli
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch

More Information

UniProt
P17661 Related reagents
Entrez Gene
DES Related reagents
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of Desmin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2 ug/ml
Immunohistology - Frozen 1/100 1/500
Immunohistology - Paraffin 1 1/100 1/500
Western Blotting 1/200 1/2000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB