Desmin antibody | AbD03744

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Anti Human Desmin Antibody, clone AbD03744 thumbnail image 1

Anti Human Desmin Antibody, clone AbD03744 thumbnail image 2

Anti Human Desmin Antibody, clone AbD03744 gallery image 1 — Staining of paraffin processed normal human appendix with Human anti Human desmin (**HCA023**).

Anti Human Desmin Antibody, clone AbD03744 gallery image 2 — Western blot analysis of C2C12 whole cell lysate probed with Human anti Desmin (**HCA023**) at 1/200 (2.5μg/ml) followed by detection with HRP conjugated Mouse anti Histidine tag (MCA1396P ) at 1/1000 (1μg/ml). Arrow points to Desmin (molecular weight 55kDa).

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Human anti Human Desmin

Product Type: Monoclonal Antibody
Clone: AbD03744
Isotype: HuCAL Fab bivalent
Specificity: Desmin

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Product Code Applications Pack Size List Price Your Price Qty

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HCA023 Datasheet Datasheet SDS SDS	C E P * WB	0.1 mg		Log in	Get Quote
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Human anti Human desmin, clone AbD03744 recognizes desmin, a member of the class III family of intermediate filaments. Desmin constitutes part of the cytoskeleton, and is the characteristic intermediate filament of all three types of muscle cells (skeletal, cardiac and smooth muscle).

Desmin is a 52 kDa protein encoded by nine exons of a gene located at 2q35. Desmin forms a cytoskeletal network across the muscle fiber bordering at the plasma and nuclear membrane and is particularly localized to the subplasmalemmal region and the Z-band. Staining for desmin is useful for identification of rhabdomyosarcomas and leiomyomas.

For use in immunohistology staining we recommend the use of an anti-His tag bridging antibody, followed by visualization using the Dako Duet staining system.

Target Species: Human
Product Form: A bivalent human recombinant Fab selected from the HuCAL GOLD phage display library. Expressed in E. Coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Preparation: Metal chelate affinity chromatography
Source: E.coli
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Recombinant human desmin produced in E. coli.
Approx. Protein Concentrations: Antibody concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 6 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		2 ug/ml
Immunohistology - Frozen	1/100	1/500
Immunohistology - Paraffin ¹	1/100	1/500
Western Blotting	1/200	1/2000

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description	Product Code	Applications	Pack Size	Your Price
Mouse anti Penta Histidine Tag:HRP	MCA5995P	E WB	125 µl	Log in	Get Quote
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Description	Mouse anti Penta Histidine Tag:HRP

RRID: AB_770095
UniProt: P17661
Entrez Gene: DES
Licensed Use: For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.