DDX48 antibody | AB02/3F4
Mouse anti DDX48 antibody recognizes eukaryotic initiation factor 4A-III, also known as eIF-4A-III (EIF4A3) and NMP 265.
DDX48 is a core component of exon junction complexes (EJCs) which act as molecular markers for post-transcriptional gene regulation (Ryu et al. 2019). Specifically, DDX48 regulates the exon junction complex and nonsense-mediated mRNA decay during processing of mRNA transcripts. DDX48 mutations have been identified in Richieri-Costa-Pereira syndrome, characterized by craniofacial anomalies and severe limb defects (Mazloomian et al. 2019). Accordingly, the 5' untranslated region of DDX48 has been identified as an essential factor for craniofacial and neural development (Hsia et al. 2018). DDX48 dysfunction has been identified in cancer, overexpressed at the transcriptional level in several human malignancies. This result is consistent with the known regulation of apoptosis and the cell cycle by DDX48 via several cancer-related pathways (Lin et al. 2018).
DDX48 is a core component of exon junction complexes (EJCs) which act as molecular markers for post-transcriptional gene regulation (Ryu et al. 2019). Specifically, DDX48 regulates the exon junction complex and nonsense-mediated mRNA decay during processing of mRNA transcripts. DDX48 mutations have been identified in Richieri-Costa-Pereira syndrome, characterized by craniofacial anomalies and severe limb defects (Mazloomian et al. 2019). Accordingly, the 5' untranslated region of DDX48 has been identified as an essential factor for craniofacial and neural development (Hsia et al. 2018). DDX48 dysfunction has been identified in cancer, overexpressed at the transcriptional level in several human malignancies. This result is consistent with the known regulation of apoptosis and the cell cycle by DDX48 via several cancer-related pathways (Lin et al. 2018).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti DDX48 antibody detects a band of approximately 46 kDa in HeLa cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-411 of human DDX48
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P38919
- Entrez Gene
- EIF4A3
- GO Terms
- GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
- GO:0005515 protein binding
- GO:0004004 ATP-dependent RNA helicase activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0005681 spliceosomal complex
- GO:0006364 rRNA processing
- GO:0008143 poly(A) RNA binding
- GO:0006397 mRNA processing
- GO:0008380 RNA splicing
- GO:0045727 positive regulation of translation
- GO:0016607 nuclear speck
- GO:0017148 negative regulation of translation
- GO:0051028 mRNA transport
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of DDX48 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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