DDX48 antibody | AB02/3F4

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Mouse anti DDX48

Product Type
PrecisionAb Monoclonal
Clone
AB02/3F4
Isotype
IgG2a
Format
Purified
Product Code Applications Pack Size List Price Quantity
VMA00796
Datasheet
WB
SDS
100 µl loader

Mouse anti DDX48 antibody recognizes eukaryotic initiation factor 4A-III, also known as eIF-4A-III (EIF4A3) and NMP 265.

DDX48 is a core component of exon junction complexes (EJCs) which act as molecular markers for post-transcriptional gene regulation (Ryu et al. 2019). Specifically, DDX48 regulates the exon junction complex and nonsense-mediated mRNA decay during processing of mRNA transcripts. DDX48 mutations have been identified in Richieri-Costa-Pereira syndrome, characterized by craniofacial anomalies and severe limb defects (Mazloomian et al. 2019). Accordingly, the 5' untranslated region of DDX48 has been identified as an essential factor for craniofacial and neural development (Hsia et al. 2018). DDX48 dysfunction has been identified in cancer, overexpressed at the transcriptional level in several human malignancies. This result is consistent with the known regulation of apoptosis and the cell cycle by DDX48 via several cancer-related pathways (Lin et al. 2018).

Product Details

Target Species
Human
Western Blotting
Mouse anti DDX48 antibody detects a band of approximately 46 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-411 of human DDX48
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
Guarantee
12 months from date of despatch

More Information

UniProt
P38919
Entrez Gene
EIF4A3
GO Terms
GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
GO:0005515 protein binding
GO:0004004 ATP-dependent RNA helicase activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0005681 spliceosomal complex
GO:0006364 rRNA processing
GO:0008143 poly(A) RNA binding
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0045727 positive regulation of translation
GO:0016607 nuclear speck
GO:0017148 negative regulation of translation
GO:0051028 mRNA transport
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of DDX48 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml

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