Cytochrome P450 3A4 antibody

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Mouse anti Cytochrome P450 3A4

Cytochrome P450 3A4 antibody recognizes the cytochrome P450 3A4, also known as 1,8-cineole 2-exo-monooxygenase, albendazole monooxygenase, albendazole sulfoxidase, or taurochenodeoxycholate 6-alpha-hydroxylase.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00117 WB 100 µl
Mouse anti Human cytochrome P450 3A4 antibody recognizes cytochrome P450 3A4, also known as 1,8-cineole 2-exo-monooxygenase, P450-III, steroid inducible, albendazole monooxygenase, albendazole sulfoxidase, cytochrome P450 3A3, cytochrome P450 HLp, cytochrome P450 NF-25, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450-PCN1, glucocorticoid-inducible P450, nifedipine oxidase, quinine 3-monooxygenase and taurochenodeoxycholate 6-alpha-hydroxylase.

Encoded by the CYP3A4 gene, cytochrome P450 3A4 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. CYP3A4 is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Feb 2011).

Mouse anti Human cytochrome P450 3A4 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cytochrome P450 3A4 detects a band of approximately 50 kDa in human liver tissue cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified recombinant fragment of human cytochrome P450 3A4 expressed in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P08684 Related reagents
Entrez Gene
CYP3A4 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0016021 integral to membrane
GO:0005496 steroid binding
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006706 steroid catabolic process
GO:0006805 xenobiotic metabolic process
GO:0008209 androgen metabolic process
GO:0009822 alkaloid catabolic process
GO:0009986 cell surface
GO:0019825 oxygen binding
GO:0016098 monoterpenoid metabolic process
GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
GO:0019899 enzyme binding
GO:0020037 heme binding
GO:0030343 vitamin D3 25-hydroxylase activity
GO:0033780 taurochenodeoxycholate 6alpha-hydroxylase activity
GO:0034875 caffeine oxidase activity
GO:0042359 vitamin D metabolic process
GO:0042738 exogenous drug catabolic process
GO:0046483 heterocycle metabolic process
GO:0047638 albendazole monooxygenase activity
GO:0050591 quinine 3-monooxygenase activity
GO:0050649 testosterone 6-beta-hydroxylase activity
GO:0070576 vitamin D 24-hydroxylase activity
GO:0070989 oxidative demethylation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytochrome P450 3A4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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