Cytochrome P450 3A4 antibody

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Anti Cytochrome P450 3A4 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Mouse anti Human cytochrome P450 3A4 antibody (**VMA00117**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 80 second exposure. Arrow points to cytochrome P450 3A4 (molecular weight 50 kDa).

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Mouse anti Cytochrome P450 3A4

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: Cytochrome P450 3A4

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VMA00117 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human cytochrome P450 3A4 antibody recognizes cytochrome P450 3A4, also known as 1,8-cineole 2-exo-monooxygenase, P450-III, steroid inducible, albendazole monooxygenase, albendazole sulfoxidase, cytochrome P450 3A3, cytochrome P450 HLp, cytochrome P450 NF-25, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4, cytochrome P450-PCN1, glucocorticoid-inducible P450, nifedipine oxidase, quinine 3-monooxygenase and taurochenodeoxycholate 6-alpha-hydroxylase.

Encoded by the CYP3A4 gene, cytochrome P450 3A4 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. CYP3A4 is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified (provided by RefSeq, Feb 2011).

Mouse anti Human cytochrome P450 3A4 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cytochrome P450 3A4 detects a band of approximately 50 kDa in human liver tissue cell lysates
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Purified recombinant fragment of human cytochrome P450 3A4 expressed in E. coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P08684
Entrez Gene: CYP3A4
GO Terms: GO:0009055 electron carrier activity; GO:0016021 integral to membrane; GO:0005496 steroid binding; GO:0005789 endoplasmic reticulum membrane; GO:0005792 microsome; GO:0006706 steroid catabolic process; GO:0006805 xenobiotic metabolic process; GO:0008209 androgen metabolic process; GO:0009822 alkaloid catabolic process; View More GO Terms; GO:0009986 cell surface; GO:0019825 oxygen binding; GO:0016098 monoterpenoid metabolic process; GO:0016712 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; GO:0019899 enzyme binding; GO:0020037 heme binding; GO:0030343 vitamin D3 25-hydroxylase activity; GO:0033780 taurochenodeoxycholate 6alpha-hydroxylase activity; GO:0034875 caffeine oxidase activity; GO:0042359 vitamin D metabolic process; GO:0042738 exogenous drug catabolic process; GO:0046483 heterocycle metabolic process; GO:0047638 albendazole monooxygenase activity; GO:0050591 quinine 3-monooxygenase activity; GO:0050649 testosterone 6-beta-hydroxylase activity; GO:0070576 vitamin D 24-hydroxylase activity; GO:0070989 oxidative demethylation