Cytochrome P450 2E1 antibody | OTI5F11

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Mouse anti Cytochrome P450 2E1

Cytochrome P450 2E1 antibody recognizes cytochrome P450 2E1, also known as 4-nitrophenol 2-hydroxylase, CYPIIE1, subfamily IIE (ethanol-inducible), cytochrome P450-J, flavoprotein-linked monooxygenase or microsomal monooxygenase.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI5F11
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00156 WB 100 µl
Mouse anti Human cytochrome P450 2E1 antibody recognizes cytochrome P450 2E1, also known as 4-nitrophenol 2-hydroxylase, CYPIIE1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, cytochrome P450-J, flavoprotein-linked monooxygenase, microsomal monooxygenase and xenobiotic monooxygenase.

Encoded by the CYP2E1 gene, cytochrome P450 2E1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer (provided by RefSeq, Jul 2008).

Mouse anti Human cytochrome P450 2E1 antibody detects a band of 57 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti Cytochrome P450 2E1 detects a band of approximately 57 kDa in Human liver cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human cytochrome P450 2E1 (NP_000764) produced in HEK293T cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P05181 Related reagents
Entrez Gene
CYP2E1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005792 microsome
GO:0055114 oxidation-reduction process
GO:0006805 xenobiotic metabolic process
GO:0008202 steroid metabolic process
GO:0019825 oxygen binding
GO:0016098 monoterpenoid metabolic process
GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen
GO:0019899 enzyme binding
GO:0017144 drug metabolic process
GO:0020037 heme binding
GO:0046483 heterocycle metabolic process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Cytochrome P450 2E1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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