Cystatin C antibody | E01-8H8
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E ResetMouse anti Human Cystatin C:Biotin
- Product Type
- Monoclonal Antibody
- Clone
- E01-8H8
- Isotype
- IgG1
- Specificity
- Cystatin C
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| Mouse anti Human cystatin C antibody, clone E01-8H8 , recognizes cystatin C, a small non-glycosylated 13.3 kDa basic cysteine inhibitor and a member of the Cystatin superfamily. Cystatin C is widely expressed in all nucleated cells in the body. It is also secreted from cells into circulation and other biological fluids including urine, seminal fluid, saliva and cerebrospinal fluid (Mussap & Plebani 2004). Cystatin C specifically inhibits cysteine proteases such as papain, legumain and the cathepsins B, H, K, L and S. Through the regulation of cysteine proteases, it plays a role in various biological processes including immune responses, protein turnover, phatocytosis, antigen presentation and apoptosis (Zi & Xu 2018). When released into the blood, cystatin C is filtered by the glomerous and metabolised by proximal tubular cells. Because of this filtration of cystatin C by the kidneys, this inhibitor is commonly used clinically as a biomarker of kidney function. (Zi & Xu 2018). Within immunological processes, cystatin C regulates proteolytic processing of pro-granzymes, MHC II antigen presentation and maturation of dendritic cells (Pierre & Mellman 1998). Cystatin C is encoded by the gene CST3 and mutations of this gene are responsible for the heritable disease, cerebral amyloid angiopathy (Jurczak et al. 2016). The biotinylated Mouse anti Human cystatin C antibody, clone E01-8H8 (MCA6227B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human cystatin C antibody, clone B01-8B1 (MCA6226GA) as the capture antibody. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- E. coli derived recombinant human full length cystatin C (Ser27-Ala146)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1.0 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Human Cystatin C | MCA6226GA | E | 0.1 mg |
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| Description | Mouse anti Human Cystatin C | ||||||
- UniProt
- P01034
- Entrez Gene
- CST3
- GO Terms
- GO:0001540 beta-amyloid binding
- GO:0002020 protease binding
- GO:0006952 defense response
- GO:0004869 cysteine-type endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0010703 negative regulation of histolysis
- GO:0010711 negative regulation of collagen catabolic process
- GO:0010716 negative regulation of extracellular matrix disassembly
- GO:0043206 fibril organization
- View More GO Terms
- GO:0060311 negative regulation of elastin catabolic process
- GO:0060313 negative regulation of blood vessel remodeling
View more products with Cystatin C specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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