Cyclophilin A antibody | AbD00794
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WB ResetHuman anti Cyclophilin A
- Product Type
- Monoclonal Antibody
- Clone
- AbD00794
- Isotype
- HuCAL Fab bivalent
- Specificity
- Cyclophilin A
Antibody Quality and Performance Guarantee
| Human anti cyclophilin A antibody, clone AbD00794 recognizes cyclophilin A, also known as peptidyl-prolyl cis/trans isomerase A, PPIA or rotamase A. Cyclophilin A is a ~18 kDa, ubiquitously distributed intracellular protein that can be secreted by cells in response to inflammatory stimuli (Jin et al. 2000). Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and, therefore, are believed to be involved in protein folding and/or intracellular protein transport (Fischer et al. 1989). This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions (Franke et al. 1994). Cyclophilin is a specific high-affinity binding protein for the immunosuppressant agent cyclosporin A (Handschumacher et al. 1984). Because of its dramatic effects on decreasing morbidity and increasing survival rates in human transplants, the molecular mechanism of immunosuppression by cyclosporin A has been a matter of much interest (Durette et al. 1988). Multiple PPIA pseudogenes that map to different chromosomes have been reported. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed (Chatellard-Gruaz D et al. 1994). Human anti cyclophilin antibody, clone AbD00794 has been used successfully for the detection of cyclophilin A in HeLa cell lysates by Western blotting. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Pig Expected from Sequence Monkey Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
- Preparation
- Metal-chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Full length human recombinant cyclophilin A, amino acid residues 1-164, molecular weight 17.9 kDa
- Activity
- Activity was tested by indirect ELISA: recombinant purified Cyclophilin A (5μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA005 (2μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background.
- Purity
- Purity was tested by SDS-PAGE and Coomassie-staining of a 2μg sample.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 6 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2ug/ml | |
| Western Blotting | |
0.5ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:Alk. Phos. | ||||||
| Mouse anti c-Myc:HRP | MCA2200P | C E P WB * | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti c-Myc:HRP | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
- Synonyms
- CYPA
- RRID
- AB_609613
- UniProt
- P62937
- Entrez Gene
- PPIA
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0002576 platelet degranulation
- GO:0006457 protein folding
- GO:0003755 peptidyl-prolyl cis-trans isomerase activity
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006278 RNA-dependent DNA replication
- GO:0019047 provirus integration
- View More GO Terms
- GO:0019061 uncoating of virus
- GO:0030168 platelet activation
- GO:0030260 entry into host cell
- GO:0042277 peptide binding
- GO:0045069 regulation of viral genome replication
- GO:0046790 virion binding
- GO:0050900 leukocyte migration
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA005
0710
0710R
150860
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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