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Cyclophilin A antibody | AbD00794

Human anti Cyclophilin A

Product Type
Monoclonal Antibody
HuCAL Fab bivalent
Cyclophilin A

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price

Human anti cyclophilin A antibody, clone AbD00794 recognizes cyclophilin A, also known as peptidyl-prolyl cis/trans isomerase A, PPIA or rotamase A. Cyclophilin A is a ~18 kDa, ubiquitously distributed intracellular protein that can be secreted by cells in response to inflammatory stimuli (Jin et al. 2000).

Cyclophilin A is a member of the immunophilin class of proteins that all possess peptidyl-prolyl cis/trans isomerase activity and, therefore, are believed to be involved in protein folding and/or intracellular protein transport (Fischer et al. 1989). This protein can interact with several HIV proteins including p55 gag, Vpr, and capsid protein. It has been shown to be necessary for the formation of infectious HIV virions (Franke et al. 1994). Cyclophilin is a specific high-affinity binding protein for the immunosuppressant agent cyclosporin A (Handschumacher et al. 1984). Because of its dramatic effects on decreasing morbidity and increasing survival rates in human transplants, the molecular mechanism of immunosuppression by cyclosporin A has been a matter of much interest (Durette et al. 1988). Multiple PPIA pseudogenes that map to different chromosomes have been reported. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed (Chatellard-Gruaz D et al. 1994).

Human anti cyclophilin antibody, clone AbD00794 has been used successfully for the detection of cyclophilin A in HeLa cell lysates by Western blotting.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Pig Expected from Sequence
Monkey Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Metal-chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Full length human recombinant cyclophilin A, amino acid residues 1-164, molecular weight 17.9 kDa
Activity was tested by indirect ELISA: recombinant purified Cyclophilin A (5μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA005 (2μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background.
Purity was tested by SDS-PAGE and Coomassie-staining of a 2μg sample.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
6 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml
Western Blotting 0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

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Entrez Gene
GO Terms
GO:0051082 unfolded protein binding
GO:0002576 platelet degranulation
GO:0006457 protein folding
GO:0003755 peptidyl-prolyl cis-trans isomerase activity
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006278 RNA-dependent DNA replication
GO:0019047 provirus integration
GO:0019061 uncoating of virus
GO:0030168 platelet activation
GO:0030260 entry into host cell
GO:0042277 peptide binding
GO:0045069 regulation of viral genome replication
GO:0046790 virion binding
GO:0050900 leukocyte migration
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.


0710 0710R 150860

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