Cyclin B1 antibody | RM281

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Rabbit anti Cyclin B1

Product Type
Monoclonal Antibody
Clone
RM281
Isotype
IgG
Product Code Applications Pack Size List Price Quantity
MCA6321
Datasheet
P WB
SDS
0.1 ml loader

Rabbit anti Human cyclin B1 antibody recognizes G2/mitotic-specific cyclin-B1, also known as CCNB1. Cyclins are protein kinases required for cell cycle progression, and different cyclins execute their functions at different stages of the cell cycle by binding to cyclin-dependent kinases (CDKs). Cyclin B1 forms a complex with CDK1 and regulates entry into mitosis at the G2/M transition, synchronizing critical events such as nuclear envelope breakdown and centrosome separation (Wang et al. 2014). Cyclin B1 and CDK1 are present in the mitochondria of mammalian cells, and their expression increases under conditions of DNA damage (Xie et al. 2019). Overexpression of cyclin B1 in solid tumors has been associated with worse prognosis of lung cancer and esophageal cancer, but better prognosis of colorectal cancer (Ye et al. 2016). Cyclin B1 intracts with BRCA1, which when mutated, carries a substantial risk of breast and ovarian cancer. Targeting Cyclin B1 has been suggested for BRCA1-associated breast cancer therapy (Choi et al. 2018).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
Immunogen
A peptide corresponding to cyclin B1

Storage Information

Storage
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch

More Information

UniProt
P14635
Entrez Gene
CCNB1
GO Terms
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000236 mitotic prometaphase
GO:0000922 spindle pole
GO:0000942 condensed nuclear chromosome outer kinetochore
GO:0005829 cytosol
GO:0005654 nucleoplasm
GO:0005815 microtubule organizing center
GO:0007080 mitotic metaphase plate congression
GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0043148 mitotic spindle stabilization
GO:0045931 positive regulation of mitotic cell cycle
GO:0051301 cell division
GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore
GO:0071174 mitotic cell cycle spindle checkpoint
Regulatory
For research purposes only

Applications of Cyclin B1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1/1000 1/2000
Western Blotting 1/1000 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

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