Cyclin B1 antibody | RM281
Rabbit anti Cyclin B1
- Product Type
- Monoclonal Antibody
- Clone
- RM281
- Isotype
- IgG
- Specificity
- Cyclin B1
Antibody Quality and Performance Guarantee
| Rabbit anti Human cyclin B1 antibody recognizes G2/mitotic-specific cyclin-B1, also known as CCNB1. Cyclins are protein kinases required for cell cycle progression, and different cyclins execute their functions at different stages of the cell cycle by binding to cyclin-dependent kinases (CDKs). Cyclin B1 forms a complex with CDK1 and regulates entry into mitosis at the G2/M transition, synchronizing critical events such as nuclear envelope breakdown and centrosome separation (Wang et al.. 2014). Cyclin B1 and CDK1 are present in the mitochondria of mammalian cells, and their expression increases under conditions of DNA damage (Xie et al.. 2019). Overexpression of cyclin B1 in solid tumors has been associated with worse prognosis of lung cancer and esophageal cancer, but better prognosis of colorectal cancer (Ye et al.. 2016). Cyclin B1 interacts with BRCA1, which when mutated, carries a substantial risk of breast and ovarian cancer. Targeting Cyclin B1 has been suggested for BRCA1-associated breast cancer therapy (Choi et al.. 2018). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- A peptide corresponding to cyclin B1
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
1/1000 | 1/2000 |
| Western Blotting | |
1/1000 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Sheep anti Rabbit IgG:RPE | ||||||
- UniProt
- P14635
- Entrez Gene
- CCNB1
- GO Terms
- GO:0000082 G1/S transition of mitotic cell cycle
- GO:0000086 G2/M transition of mitotic cell cycle
- GO:0000236 mitotic prometaphase
- GO:0000922 spindle pole
- GO:0000942 condensed nuclear chromosome outer kinetochore
- GO:0005829 cytosol
- GO:0005654 nucleoplasm
- GO:0005815 microtubule organizing center
- GO:0007080 mitotic metaphase plate congression
- View More GO Terms
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0043148 mitotic spindle stabilization
- GO:0045931 positive regulation of mitotic cell cycle
- GO:0051301 cell division
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
- GO:0051987 positive regulation of attachment of spindle microtubules to kinetochore
- GO:0071174 mitotic cell cycle spindle checkpoint
MCA6321
155791
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