CUL4B antibody | OTI1C4

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Anti CUL4B Antibody, clone OTI1C4 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CUL4B Antibody, clone OTI1C4 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CUL4B antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 70 second exposure. Arrow points to CUL4B (molecular weight 104 kDa)

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Mouse anti CUL4B

Product Type: PrecisionAb Monoclonal
Clone: OTI1C4
Isotype: IgG2b
Format: Purified
Specificity: CUL4B

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Mouse anti Human CUL4B antibody recognizes CUL4B, also known as cullin-4B.

CUL4B is a member of the cullin family. Encoded by the CUL4B gene, cullin-4B forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for CUL4B (provided by RefSeq, Jul 2008).

Mouse anti Human CUL4B antibody detects a band of 104 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti CUL4B detects a band of approximately 104 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Recombinant protein fragment corresponding to aa 1-300 of human CUL4B (NP_003579) produced in E.coli
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.