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Complement C5 antibody | AbD36700

Human anti Human Complement C5

Product Type
Monoclonal Antibody
Fab antibody
Complement C5

Product Code Applications Pack Size List Price Your Price Qty
New TZA034
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Human Anti-Human Complement C5 Antibody, clone AbD36700 specifically recognizes the human complement factor 5 (C5).

The complement factor 5 (C5) forms part of the complement system and the body’s innate immune system. The complement system is vital to the body’s control of inflammation, homeostasis and defense against foreign pathogens. Downstream, C5 is cleaved into C5a and C5b by the C5 convertase. C5a increases blood vessel permeability and attracts inflammatory cells, while C5b binds with other complement components to form a membrane attack complex (MAC) to kill the pathogen.

This TrailBlazer Antibody is available in two different formats: as an unconjugated monovalent Fab format (TZA034) and as a bivalent Fab antibody conjugated to HRP (TZA034P). The monovalent recombinant Fab antibody has a SpyTag incorporated, which is used to create the bivalent format by coupling two SpyTagged Fab antibodies to an HRP conjugated BiSpyCatcher2 (TZC002P). The monovalent Fab format of this Human anti-Human Complement C5 Antibody (TZA034) can also be converted by the end user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

This antibody recognizes free human C5 as well as human C5 in complex with the humanized monoclonal antibody drug eculizumab. Either format can be used to detect the human complement factor C5 in direct or indirect ELISA or to develop a pharmacokinetic (PK) antigen capture assay to measure free human complement C5 captured via immobilized Human Anti-Eculizumab Antibody, clone AbD32334 (HCA339).
20th Anniversary

Target Species
Product Form
A monovalent human recombinant Fab (kappa light chain) in the Fab-F-Spy2-H format selected from the HuCAL® phage display library and expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Human Complement C5
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD36700ad can be used in indirect ELISA to detect the human complement factor C5 or the eculizumab/C5 complex. It can also be used as detection antibody for the complex of eculizumab and human complement C5 in a PK bridging ELISA together with clone AbD32334 (HCA339) as the capture antibody.

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Eculizumab (Drug/Target Complex) HCA339 E 0.1 mg loader
List Price Your Price
Description Human anti Eculizumab (Drug/Target Complex)
Human anti Human Complement C5:HRP TZA034P E 0.1 mg
List Price Your Price
Description Human anti Human Complement C5:HRP
BiSpyCatcher2:Biotin TZC002B IY 1 mg
List Price Your Price
Description BiSpyCatcher2:Biotin
BiSpyCatcher2:HRP TZC002P IY 1 mg
List Price Your Price
Description BiSpyCatcher2:HRP

Entrez Gene
GO Terms
GO:0000187 activation of MAPK activity
GO:0004866 endopeptidase inhibitor activity
GO:0005579 membrane attack complex
GO:0005615 extracellular space
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006957 complement activation, alternative pathway
GO:0006958 complement activation, classical pathway
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0008009 chemokine activity
GO:0019835 cytolysis
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010760 negative regulation of macrophage chemotaxis
GO:0090197 positive regulation of chemokine secretion



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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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