Cebpb antibody | L01/5C4

Mouse anti Human Cebpb

Product Type
PrecisionAb Monoclonal
Clone
L01/5C4
Isotype
IgG1
Format
Purified
Specificity
Cebpb

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Mouse anti Human CEBPB antibody recognizes CCAAT/enhancer-binding protein beta, also known as liver activator protein, nuclear factor NF-IL6 or transcription factor 5.

CEBPB is a transcription factor that plays an important role in the regulation of genes involved in immune and inflammatory responses. CEBPB forms heterodimers with CEBPA and CEBPG and has also been shown to be crucial for maintenance of normal macrophage function, including macrophage-mediated muscle repair (Ruffell et al. 2009). In glioblastoma, increased activity of EGF R has been shown to result in up-regulation of CEBPB and vice versa, boosting oncogenic signaling (Selagea et al. 2016).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti Human CEBPB antibody detects bands of 40 kDa and 44 kDa in U-87 MG cell lysates
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 1-345 of human CEBPB
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation 2.5 µg
Western Blotting 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type

UniProt
P17676
Entrez Gene
CEBPB
GO Terms
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0006953 acute-phase response
GO:0006955 immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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