CDK12 antibody | 45F7-H2

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Anti CDK12 Antibody, clone 45F7-H2 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti CDK12 Antibody, clone 45F7-H2 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with CDK12 antibody (1/1,000, **VMA00874**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP

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Mouse anti CDK12

Product Type: PrecisionAb Monoclonal
Clone: 45F7-H2
Isotype: IgG1
Format: Purified
Specificity: CDK12

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VMA00874 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Mouse anti Human CDK12 antibody recognizes cyclin-dependent kinase 12 (CDK12), otherwise known as CrkRS and CRK7

CDK12 is a member of the CDK family of serine/threonine protein kinases which can be divided into two groups, those that regulate the cell cycle and those that regulate transcription (Paculová and Kohoutek 2017). CDK12 is a regulator of transcription, and can upregulate the expression of genes involved with responses to DNA damage, stress, and heat shock (Lui et al. 2018). CDK proteins bind to cyclin subunits to activate their functions. CDK12, like CDK13, forms a complex with cyclin K to regulate several cellular processes (Kohoutek and Blazek 2012). Genomic alterations in CDK12 have been identified with a frequency of 5 to 15% in various cancer types including esophageal, stomach, breast, endometrial, uterine, ovarian, bladder, colorectal, and pancreatic cancers. CDK12 inhibition has been suggested as a target to slow cancer growth (Lui et al. 2018).

Target Species: Human
Western Blotting: Mouse anti CDK12 detects a band of approximately 203 kDa in HEK293 cell lysates
Product Form: Purified IgG - Liquid
Preparation: Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
Immunogen: A peptide corresponding to amino acid 479-508 of CDK12
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C. avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt: Q9NYV4
Entrez Gene: CDK12
GO Terms: GO:0005515 protein binding; GO:0003702 RNA polymerase II transcription factor activity; GO:0004693 cyclin-dependent protein kinase activity; GO:0005524 ATP binding; GO:0005730 nucleolus; GO:0016607 nuclear speck; GO:0046777 protein autophosphorylation