CDK12 antibody | 45F7-H2

Mouse anti CDK12

Product Type
PrecisionAb Monoclonal
Clone
45F7-H2
Isotype
IgG1
Format
Purified
Specificity
CDK12

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VMA00874
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Mouse anti Human CDK12 antibody recognizes cyclin-dependent kinase 12 (CDK12), otherwise known as CrkRS and CRK7

CDK12 is a member of the CDK family of serine/threonine protein kinases which can be divided into two groups, those that regulate the cell cycle and those that regulate transcription (Paculová and Kohoutek 2017). CDK12 is a regulator of transcription, and can upregulate the expression of genes involved with responses to DNA damage, stress, and heat shock (Lui et al. 2018). CDK proteins bind to cyclin subunits to activate their functions. CDK12, like CDK13, forms a complex with cyclin K to regulate several cellular processes (Kohoutek and Blazek 2012). Genomic alterations in CDK12 have been identified with a frequency of 5 to 15% in various cancer types including esophageal, stomach, breast, endometrial, uterine, ovarian, bladder, colorectal, and pancreatic cancers. CDK12 inhibition has been suggested as a target to slow cancer growth (Lui et al. 2018).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti CDK12 detects a band of approximately 203 kDa in HEK293 cell lysates
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
A peptide corresponding to amino acid 479-508 of CDK12
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C. avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
Q9NYV4
Entrez Gene
CDK12
GO Terms
GO:0005515 protein binding
GO:0003702 RNA polymerase II transcription factor activity
GO:0004693 cyclin-dependent protein kinase activity
GO:0005524 ATP binding
GO:0005730 nucleolus
GO:0016607 nuclear speck
GO:0046777 protein autophosphorylation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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