CD97 antibody | MEM-180

Mouse anti Human CD97

Product Type
Monoclonal Antibody
Clone
MEM-180
Isotype
IgG1
Specificity
CD97

Product Code Applications Pack Size List Price Your Price Qty
MCA1878
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD97 antibody, clone MEM-180 recognizes the human CD97 cell surface antigen, a 74-89 kDa glycoprotein expressed by granulocytes, monocytes, some activated lymphocytes and weakly by resting lymphocytes (5-15%). Mouse anti Human CD97 antibody, clone MEM-180 has been reported to detect CD97 antigen weakly in western blotting.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/400
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood

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References for CD97 antibody

  1. Steinert, M. et al. (2002) Expression and regulation of CD97 in colorectal carcinoma cell lines and tumor tissues.
    Am J Pathol. 161 (5): 1657-67.
  2. Hamann, J. et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.
    Eur J Immunol. 28 (5): 1701-7.
  3. Wobus, M. et al. (2004) N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding.
    Int J Cancer. 112 (5): 815-22.
  4. Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
    FASEB J. 22 (3): 741-51.
  5. Wobus, M. et al. (2015) Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia.
    Oncotarget. 6 (36): 38804-15.

RRID
AB_323206
UniProt
P48960
Entrez Gene
CD97
GO Terms
GO:0005515 protein binding
GO:0005509 calcium ion binding
GO:0007155 cell adhesion
GO:0004930 G-protein coupled receptor activity
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0006928 cellular component movement
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007218 neuropeptide signaling pathway
GO:0007267 cell-cell signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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