CD97 antibody | MEM-180
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F ResetMouse anti Human CD97
- Product Type
- Monoclonal Antibody
- Clone
- MEM-180
- Isotype
- IgG1
- Specificity
- CD97
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD97 antibody, clone MEM-180 recognizes the human CD97 cell surface antigen, a 74-89 kDa glycoprotein expressed by granulocytes, monocytes, some activated lymphocytes and weakly by resting lymphocytes (5-15%). Mouse anti Human CD97 antibody, clone MEM-180 has been reported to detect CD97 antigen weakly in western blotting. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/200 | 1/400 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD97 antibody
-
Steinert, M. et al. (2002) Expression and regulation of CD97 in colorectal carcinoma cell lines and tumor tissues.
Am J Pathol. 161 (5): 1657-67. -
Hamann, J. et al. (1998) Characterization of the CD55 (DAF)-binding site on the seven-span transmembrane receptor CD97.
Eur J Immunol. 28 (5): 1701-7. -
Wobus, M. et al. (2004) N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding.
Int J Cancer. 112 (5): 815-22. -
Yona, S. et al. (2008) Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function.
FASEB J. 22 (3): 741-51. -
Wobus, M. et al. (2015) Association of the EGF-TM7 receptor CD97 expression with FLT3-ITD in acute myeloid leukemia.
Oncotarget. 6 (36): 38804-15.
- RRID
- AB_323206
- UniProt
- P48960
- Entrez Gene
- CD97
- GO Terms
- GO:0005515 protein binding
- GO:0005509 calcium ion binding
- GO:0007155 cell adhesion
- GO:0004930 G-protein coupled receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0006928 cellular component movement
- GO:0006954 inflammatory response
- GO:0006955 immune response
- View More GO Terms
- GO:0007218 neuropeptide signaling pathway
- GO:0007267 cell-cell signaling
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