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CD86 antibody | BU63

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Anti Human CD86 Antibody, clone BU63 thumbnail image 1 Anti Human CD86 Antibody, clone BU63 thumbnail image 2 Anti Human CD86 Antibody, clone BU63 thumbnail image 3 Anti Human CD86 Antibody, clone BU63 thumbnail image 4 Anti Human CD86 Antibody, clone BU63 thumbnail image 5 Anti Human CD86 Antibody, clone BU63 thumbnail image 6 Anti Human CD86 Antibody, clone BU63 thumbnail image 7
Anti Human CD86 Antibody, clone BU63 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Alexa Fluor 700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and Alexa Fluor 700 conjugated Mouse anti Human CD86 (MCA1118A700). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD86 Antibody, clone BU63 gallery image 2
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and Alexa Fluor 488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA463A700) and Alexa Fluor 488 conjugated Mouse anti Human CD86 (MCA1118A488). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD86 Antibody, clone BU63 gallery image 3
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA1568A700) and Pacific Blue conjugated Mouse IgG1 isotype control (MCA928PB). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD14 (MCA463A700) and Pacific Blue conjugated Mouse anti Human CD86 (MCA1118PB). All experiments performed on red cell lysed human blood gated on live mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD86 Antibody, clone BU63 gallery image 4
Published customer image:
FITC conjugated Mouse anti Human CD86 antibody, clone Bu63 (MCA1118F) used for phenotyping monocyte derived dendritic cells by flow cytometry.
Image caption:
Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry.
Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1α,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-α, IL-1β, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.

From: Sprater F, Hovden A-O, Appel S (2012)
Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.
PLoS ONE 7(11): e49577.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD86 Antibody, clone BU63 gallery image 5
Figure A. RPE conjugated mouse anti human HLA DP/DQ/DR (MCA477PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti human HLA DP/DQ/DR (MCA477PE) and FITC conjugated mouse anti human CD86 (MCA1118F). All experiments performed on human peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).
Anti Human CD86 Antibody, clone BU63 gallery image 6
Published customer image:
Mouse anti Human CD86 antibody, clone Bu63 (MCA1118) used for the evaluation of CD86 expression on cell populations by flow cytometry.
Image caption:
DC differentiation from H1 hESC.
Cells were harvested from cultures at various time points and analysed by flow cytometry for the onset of hematopoiesis and the appearance of DC. (e) Phenotype of immature and mature H1-DCs compared with human moDC. DCs were cultured either in medium alone or medium supplemented with the maturation cocktail and stained for MHC class II, the maturation marker CD83 and classical costimulatory molecules. Dead cells were excluded from the analysis using 7-AAD. Dashed histograms show the phenotype of immature DCs while the filled histograms represent mature DCs. Open histograms depict background staining using isotype-matched controls.

From: Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ.
Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
J Biomed Biotechnol. 2012;2012:172420.
doi: 10.1155/2012/172420.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD86 Antibody, clone BU63 gallery image 7
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (​MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (​MCA1568SBV440) and StarBright Violet 790 conjugated Mouse anti Human CD86 (​MCA1118SBV790). All experiments performed on human PBMCs gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD86:StarBright Violet 790

Product Type
Monoclonal Antibody
Clone
BU63
Isotype
IgG1
Specificity
CD86

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MCA1118SBV790
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F 100 Tests/0.5ml loader
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Mouse anti Human CD86 antibody, clone Bu63 recognizes human CD86 also known as B7-2, a type I transmembrane protein expressed by monocytes and activated B cells (Engel et al. 1994). CD86 acts as a co-stimulaory molecule along with CD80 (Lanier et al. 1995) and is a ligand for CD28 and CTLA-4 (Azuma et al. 1993).

CD86 is a member of the Immunoglobulin superfamily and carries an extracellular domain bearing both an Ig-v-like domain which contains the CTLA-4 binding site and an adjacent C2-like domain. CD86 plays an important role in co-stimulation of T cell proliferation (Freeman et al. 1993), IL-2 production (Ribot et al. 2012) and in the primary immune response (Schultze et al. 1996).

Domain depletion epitope mapping indicates that the binding site of Mouse anti Human CD86,clone Bu63 is located within the Ig-v-like domain of human CD86 (Jeanin et al. 1997).

CD86 along with CD80 may be exploited as receptors for adenovirus entry into cells (Short et al. 2004 2006).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Violet 790 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human peripheral blood lymphocytes.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3.X63 Ag8653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 790 402 782
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra

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References for CD86 antibody

  1. Goodyear, O. et al. (2010) Induction of a CD8+ T-cell response to the MAGE cancer testis antigen by combined treatment with azacitidine and sodium valproate in patients with acute myeloid leukemia and myelodysplasia.
    Blood. 116: 1908-18.
  2. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
    J Immunol. 176 (10): 5730-4.
  3. Salte, T. et al. (2010) Increased intracellular growth of Mycobacterium avium in HIV-1 exposed monocyte-derived dendritic cells.
    Microbes Infect. 13: 276-83.
  4. Adler, H.S. et al. (2010) Neuronal nitric oxide synthase modulates maturation of human dendritic cells.
    J Immunol. 184: 6025-34.
  5. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12:2.
  6. Kapsogeorgou, E.K. et al. (2001) Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4.
    J Immunol. 166: 3107-13.
  7. Lozanoska-Ochser, B. et al. (2008) Expression of CD86 on human islet endothelial cells facilitates T cell adhesion and migration.
    J Immunol. 181: 6109-16.
  8. Urban, B.C. et al. (2001) A role for CD36 in the regulation of dendritic cell function.
    Proc Natl Acad Sci U S A. 98: 8750-5.
    9. View The Latest Product References
  9. Zhan, H. et al. (2003) The immunomodulatory role of human conjunctival epithelial cells.
    Invest Ophthalmol Vis Sci. 44: 3906-10.
  10. Sprater, F. et al. (2012) Expression of ESE-3 isoforms in immunogenic and tolerogenic human monocyte-derived dendritic cells.
    PLoS One. 7 (11): e49577.
  11. McCarthy, N.E. et al. (2013) Proinflammatory Vδ2+ T Cells Populate the Human Intestinal Mucosa and Enhance IFN-γ Production by Colonic αβ T Cells.
    J Immunol. 191: 2752-63.
  12. Hofmann-Wellenhof, R. et al. (2004) Sunburn cell formation, dendritic cell migration, and immunomodulatory factor production after solar-simulated irradiation of sunscreen-treated human skin explants in vitro.
    J Invest Dermatol. 123: 781-7.
  13. Rajkovic, I. et al. (2011) Differences in T-helper polarizing capability between human monocyte-derived dendritic cells and monocyte-derived Langerhans'-like cells.
    Immunology. 132: 217-25.
  14. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  15. Ikezumi, Y. et al. (2021) Steroid treatment promotes an M2 anti-inflammatory macrophage phenotype in childhood lupus nephritis.
    Pediatr Nephrol. 36 (2): 349-59.

Flow Cytometry

Immunofluorescence

Synonyms
B7-2
UniProt
P42081
Entrez Gene
CD86
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0015026 coreceptor activity
GO:0016563 transcription activator activity
GO:0031295 T cell costimulation
GO:0043017 positive regulation of lymphotoxin A biosynthetic process
GO:0044419 interspecies interaction between organisms
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045404 positive regulation of interleukin-4 biosynthetic process
GO:0045630 positive regulation of T-helper 2 cell differentiation

MCA1118SBV790

64605632

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