CD69 antibody | FN50

Mouse anti Human CD69:StarBright Violet 670

Product Type
Monoclonal Antibody
Clone
FN50
Isotype
IgG1
Specificity
CD69

Product Code Applications Pack Size List Price Your Price Qty
MCA2806SBV670
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F 100 Tests/0.5ml loader
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Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).

CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel 1997), neutrophils, thymocytes and platelets (Gaviol et al. 1992). Expression of CD69 is rapidly induced on activation by infection or chronic inflamation (Sancho et al. 2005). Multiple dimeric glycoforms of CD69 can be formed through differential glycosylation of the monomeric subunits (Vance et al. 1997).

Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Baboon
Chimpanzee
Cynomolgus monkey
Rhesus Monkey
Macaque
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 670 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Activated human B-cells.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 670 400 667
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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References for CD69 antibody

  1. Holte, H. et al. (1989) Ki67 and 4F2 antigen expression as well as DNA synthesis predict survival at relapse/tumour progression in low-grade B-cell lymphoma.
    Int J Cancer. 44 (6): 975-80.
  2. Herberth, M. et al. (2010) Differential effects on T-cell function following exposure to serum from schizophrenia smokers.
    Mol Psychiatry. 15 (4): 364-71.
  3. Schaeuble, K. et al. (2011) Cross-talk between TCR and CCR7 signaling sets a temporal threshold for enhanced T lymphocyte migration.
    J Immunol. 187 (11): 5645-52.
  4. Sela, M. et al. (2011) Sequential phosphorylation of SLP-76 at tyrosine 173 is required for activation of T and mast cells.
    EMBO J. 30 (15): 3160-72.
  5. Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
    BMC Cancer. 11: 410.
  6. Schwitalle, Y. et al. (2004) Immunogenic peptides generated by frameshift mutations in DNA mismatch repair-deficient cancer cells.
    Cancer Immun. 4: 14.
  7. Sutavani, R.V. et al. (2013) CD55 Costimulation Induces Differentiation of a Discrete T Regulatory Type 1 Cell Population with a Stable Phenotype.
    J Immunol. 191: 5895-903.
  8. Walter, G.J. et al. (2013) Interaction with activated monocytes enhances cytokine expression and suppressive activity of human CD4+CD45ro+CD25+CD127(low) regulatory T cells.
    Arthritis Rheum. 65: 627-38.
  9. View The Latest Product References
  10. Kuric, E. et al. (2017) Demonstration of Tissue Resident Memory CD8 T Cells in Insulitic Lesions in Adult Patients with Recent-Onset Type 1 Diabetes.
    Am J Pathol. 187 (3): 581-8.
  11. Karnell, F.G. et al. (2017) Reconstitution of immune cell populations in multiple sclerosis patients after autologous stem cell transplantation.
    Clin Exp Immunol. 189 (3): 268-278.
  12. Rossatti, P. et al. (2022) Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.
    BMC Biol. 20 (1): 189.

Flow Cytometry

Synonyms
AIM
UniProt
Q07108
Entrez Gene
CD69
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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