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CD63 antibody | MEM-259

Mouse anti Human CD63:APC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price

Mouse anti Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.

CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischer et al. 1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamoto et al. 1994). CD63 expression is also associated with melanoma development (Radford et al. 1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okada et al. 1999).

Target Species
Product Form
Purified IgG conjugated to allophycocyanin (APC) - lyophilized
Reconstitute with 1ml distilled water
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
HPB-ALL cell line.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
APC 650 661
For research purposes only
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl or 100μl whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:APC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD63 antibody

  1. Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
    Mol Cell Proteomics. 6 (5): 767-80.
  2. Durand-Panteix, S. et al. (2012) B7-H1, which represses EBV-immortalized B cell killing by autologous T and NK cells, is oppositely regulated by c-Myc and EBV latency III program at both mRNA and secretory lysosome levels.
    J Immunol. 189 (1): 181-90.
  3. Abdel-Latif, D. et al. (2004) Rac2 is critical for neutrophil primary granule exocytosis.
    Blood. 104: 832-9.
  4. Norling, L.V. et al. (2012) Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.
    Arterioscler Thromb Vasc Biol. 32 (8): 1970-8.
  5. McKechnie, N.M. et al. (2006) Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles.
    Exp Eye Res. 83: 304-14.
  6. Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
    PLoS One. 8(5):e62654.
  7. Pliyev, B.K. (2008) Activated human neutrophils rapidly release the chemotactically active D2D3 form of the urokinase-type plasminogen activator receptor (uPAR/CD87).
    Mol Cell Biochem. 321: 111-22.
  8. Suraeva, N.M. et al. (2015) Changes in the Morphological and Immunological Characteristics of Mel Ibr Melanoma Cells in Response to Chicken Embryo Extract.
    Bull Exp Biol Med. 159 (4): 520-3.
  9. View The Latest Product References
  10. Schäfer, T. et al. (2010) A granular variant of CD63 is a regulator of repeated human mast cell degranulation.
    Allergy. 65 (10): 1242-55.
  11. Jakhria, T. et al. (2014) β2-microglobulin amyloid fibrils are nanoparticles that disrupt lysosomal membrane protein trafficking and inhibit protein degradation by lysosomes.
    J Biol Chem. 289 (52): 35781-94.
  12. Ethier, C. et al. (2016) Calcitriol Reduces Eosinophil Necrosis Which Leads to the Diminished Release of Cytotoxic Granules.
    Int Arch Allergy Immunol. 171 (2): 119-29.
  13. Nielsen, M.H. et al. (2023) Increased extracellular vesicles (EVs) related to T cell-mediated inflammation and vascular function in familial hypercholesterolemia.
    Atheroscler Plus. 53: 16-25.
  14. Ilarraza, R. et al. (2023) Rac2 regulates immune complex-mediated granule polarization and exocytosis in neutrophils.
    J Leukoc Biol. 114 (2): 116-125.
  15. Vrancx, C. et al. (2021) Mechanism of Cellular Formation and In Vivo Seeding Effects of Hexameric β-Amyloid Assemblies.
    Mol Neurobiol. 58 (12): 6647-69.
  16. Gratpain, V. et al. (2024) Influence of a pro-inflammatory stimulus on the miRNA and lipid content of human dental stem cell-derived extracellular vesicles and their impact on microglial activation
    Heliyon. : e27025.

Entrez Gene
GO Terms
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005765 lysosomal membrane
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031902 late endosome membrane



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