CD63 antibody | MEM-259
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Mouse anti Human CD63:APC
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.
CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischer et al. 1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamoto et al. 1994). CD63 expression is also associated with melanoma development (Radford et al. 1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okada et al. 1999).
- Target Species
- Product Form
- Purified IgG conjugated to allophycocyanin (APC) - lyophilized
- Reconstitute with 1ml distilled water
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- HPB-ALL cell line.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) APC 650 661
- For research purposes only
- 12 months from date of despatch
After reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl or 100μl whole blood
References for CD63 antibody
Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
Mol Cell Proteomics. 6 (5): 767-80.
Durand-Panteix, S. et al. (2012) B7-H1, which represses EBV-immortalized B cell killing by autologous T and NK cells, is oppositely regulated by c-Myc and EBV latency III program at both mRNA and secretory lysosome levels.
J Immunol. 189 (1): 181-90.
Abdel-Latif, D. et al. (2004) Rac2 is critical for neutrophil primary granule exocytosis.
Blood. 104: 832-9.
Norling, L.V. et al. (2012) Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.
Arterioscler Thromb Vasc Biol. 32 (8): 1970-8.
McKechnie, N.M. et al. (2006) Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles.
Exp Eye Res. 83: 304-14.
Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
PLoS One. 8(5):e62654.
Pliyev, B.K. (2008) Activated human neutrophils rapidly release the chemotactically active D2D3 form of the urokinase-type plasminogen activator receptor (uPAR/CD87).
Mol Cell Biochem. 321: 111-22.
Suraeva, N.M. et al. (2015) Changes in the Morphological and Immunological Characteristics of Mel Ibr Melanoma Cells in Response to Chicken Embryo Extract.
Bull Exp Biol Med. 159 (4): 520-3.
View The Latest Product References
Schäfer, T. et al. (2010) A granular variant of CD63 is a regulator of repeated human mast cell degranulation.
Allergy. 65 (10): 1242-55.
Jakhria, T. et al. (2014) β2-microglobulin amyloid fibrils are nanoparticles that disrupt lysosomal membrane protein trafficking and inhibit protein degradation by lysosomes.
J Biol Chem. 289 (52): 35781-94.
Ethier, C. et al. (2016) Calcitriol Reduces Eosinophil Necrosis Which Leads to the Diminished Release of Cytotoxic Granules.
Int Arch Allergy Immunol. 171 (2): 119-29.
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