CD62L antibody | FMC46
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F P ResetMouse anti Human CD62L:StarBright Violet 440
- Product Type
- Monoclonal Antibody
- Clone
- FMC46
- Isotype
- IgG2b
- Specificity
- CD62L
Mouse anti Human CD62L antibody, clone FMC46 recognizes human CD62L, also known a L-selectin, a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes. CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naïve lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains. The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Cynomolgus monkey Rhesus Monkey Dog - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 440 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20 - Immunogen
- PHA stimulated lymphoblasts
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 440 383 436 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts.
This product should be stored undiluted.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat |
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
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Description | Human Seroblock |
Source Reference
-
Pilarski, L.M. et al. (1991) FMC46, a cell protrusion-associated leukocyte adhesion molecule-1 epitope on human lymphocytes and thymocytes.
J Immunol. 147 (1): 136-43.
References for CD62L antibody
-
Zola, H. et al. (1991) The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry.
Dis Markers. 9 (2): 103-18. -
Sopp, P. & Howard, C.J. (1997) Cross-reactivity of monoclonal antibodies to defined human leucocyte differentiation antigens with bovine cells.
Vet Immunol Immunopathol. 56 (1-2): 11-25. -
Haanstra, K.G. et al. (2008) Characterization of naturally occurring CD4+CD25+ regulatory T cells in rhesus monkeys.
Transplantation 85:1185-92. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9. -
Raposo, R.A. et al. (2011) Protein Kinase C and NF-{kappa}B-Dependent CD4 Downregulation in Macrophages Induced by T Cell-Derived Soluble Factors: Consequences for HIV-1 Infection.
J Immunol. 187: 748-59. -
Hughes, S.F. et al. (2010) Total hip and knee replacement surgery results in changes in leukocyte and endothelial markers.
J Inflamm (Lond). 7:2. -
Bismarck, D. et al. (2012) Canine CD4+CD8+ double positive T cells in peripheral blood have features of activated T cells.
Vet Immunol Immunopathol. 149: 157-66. -
Hartley, A.N. & Tarleton, R.L. (2015) Chemokine receptor 7 (CCR7)-expression and IFNγ production define vaccine-specific canine T-cell subsets.
Vet Immunol Immunopathol. 164 (3-4): 127-36.
View The Latest Product References
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Hayhoe, R.P. et al. (2006) Annexin 1 and its bioactive peptide inhibit neutrophil-endothelium interactions under flow: indication of distinct receptor involvement.
Blood. 107 (5): 2123-30. -
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther. 321 (2): 656-62. -
Aspinall, A.I. et al. (2010) CX(3)CR1 and vascular adhesion protein-1-dependent recruitment of CD16(+) monocytes across human liver sinusoidal endothelium.
Hepatology. 51 (6): 2030-9. -
Rothe, K. et al. (2017) Canine peripheral blood CD4+CD8+ double-positive Tcell subpopulations exhibit distinct Tcell phenotypes and effector functions.
Vet Immunol Immunopathol. 185: 48-56. -
Withers, S.S. et al. (2018) Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs.
Dev Comp Immunol. 87: 64-74. -
Hughes, S.F. et al. (2020) The role of phagocytic leukocytes following flexible ureterenoscopy, for the treatment of kidney stones: an observational, clinical pilots-study.
Eur J Med Res. 25 (1): 68. -
Svitek, N. et al. (2018) An Ad/MVA vectored Theileria parva antigen induces schizont-specific CD8+ central memory T cells and confers partial protection against a lethal challenge.
NPJ Vaccines. 3: 35. -
Tucker, N. et al. (2023) Bovine blood and milk T-cell subsets in distinct states of activation and differentiation during subclinical Staphylococcus aureus mastitis.
J Reprod Immunol. 156: 103826. -
Yamauchi, A. et al. (2023) Negative Influence of Aging on Differentiation and Proliferation of CD8(+) T-Cells in Dogs.
Vet Sci. 10 (9): 541
- Synonyms
- LECAM-1
- L-SELECTIN
- UniProt
- P14151
- Entrez Gene
- SELL
- GO Terms
- GO:0007596 blood coagulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0008201 heparin binding
- GO:0043208 glycosphingolipid binding
- GO:0050776 regulation of immune response
- GO:0050900 leukocyte migration
MCA1076SBV440
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