CD58 antibody | MEM-63

100% Secure


Mouse anti Human CD58:APC

Mouse anti Human CD58:FITC

Product Type
Monoclonal Antibody
Clone
MEM-63
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2126APC F 100 Tests
MCA2126F F 100 Tests/2ml
Mouse anti Human CD58 antibody, clone MEM-63 recognizes human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Macgoba et al. 1989). CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Allophycocyanin (APC) - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by size-exclusion chromatography
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide

Storage Information

Storage
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P19256 Related reagents
Entrez Gene
CD58 Related reagents
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016337 cell-cell adhesion
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of CD58 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:APC MCA928APC 100 Tests F
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for CD58 antibody

  1. Makgoba, M.W. et al. (1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.
    Immunol Today. 10 (12): 417-22.
  2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
    Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716.
  3. Grundy, J.E. et al. (1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.
    Immunology. 78 (3): 413-20.
  4. Megyola, C. et al. (2011) Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.
    Virol J. 8: 84.
  5. Somasundaram, R.T. et al. (2016) Subcellular differential expression of Ep-ICD in oral dysplasia and cancer is associated with disease progression and prognosis.
    BMC Cancer. 16: 486.