CD58 antibody | MEM-63

Mouse anti Human CD58:FITC

Product Type
Monoclonal Antibody
Clone
MEM-63
Isotype
IgG1
Specificity
CD58

Product Code Applications Pack Size List Price Your Price Qty
MCA2126F
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SDS Safety Datasheet SDS
F 100 Tests/2ml loader
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Mouse anti Human CD58 antibody, clone MEM-63 recognizes human CD58, also known as LFA-3. CD58 is a membrane glycoprotein of ~55-70 kDa. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. CD58 is a heavily N-glycosylated cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989). CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD58 antibody

  1. Makgoba, M.W. et al. (1989) The CD2-LFA-3 and LFA-1-ICAM pathways: relevance to T-cell recognition.
    Immunol Today. 10 (12): 417-22.
  2. Shaw, S., Johnson, J.P., (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.
    Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr. Oxford University Press. pp 714-716.
  3. Grundy, J.E. et al. (1993) Increased adherence of CD2 peripheral blood lymphocytes to cytomegalovirus-infected fibroblasts is blocked by anti-LFA-3 antibody.
    Immunology. 78 (3): 413-20.
  4. Megyola, C. et al. (2011) Identification of a sub-population of B cells that proliferates after infection with Epstein-Barr virus.
    Virol J. 8: 84.

Synonyms
LFA-3
RRID
AB_323491
UniProt
P19256
Entrez Gene
CD58
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016337 cell-cell adhesion
GO:0050900 leukocyte migration
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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