CD52 antibody | YTH66.9HL

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Rat anti Human CD52

Product Type
Monoclonal Antibody
Clone
YTH66.9HL
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA349R C CT F WB 0.1 ml
Rat anti Human CD52 antibody, clone YTH66.9HL recognizes human CD52, also known as CAMPATH-1. The CD52 antigen is a small heavily glycosylated peptide attached to the cell surface membrane via a GPI link. The apparent molecular mass of the antigen on SDS-PAGE is 25-29 kDa.

CD52 is expressed at high density by lymphocytes, monocytes, eosinophils, thymocytes and macrophages. It is expressed by most lymphoid derived malignancies, although expression on myeloma cells is variable.

Humanised versions of CAMPATH-1 specific antibodies are used for the treatment of a range of lymphoid malignancies (Blatt et al. 2014).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig fraction - liquid
Preparation
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human T lymphocytes.
Approx. Protein Concentrations
IgM concentration 2 mg/ml
Fusion Partners
Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag.1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P31358 Related reagents
Entrez Gene
CD52 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0005624 membrane fraction
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0031225 anchored to membrane
GO:0045730 respiratory burst
Regulatory
For research purposes only

Applications of CD52 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Cytotoxic Assays
Flow Cytometry 1/20
Immunohistology - Frozen 1/20
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgM:Biotin 302008 1 mg C E IB
Goat anti Rat IgM:RPE 302009 0.5 mg F
Goat anti Rat IgM:HRP STAR116P 0.5 mg E WB

Product Specific References

References for CD52 antibody

  1. Hale, G. et al. (1985) Reactivity of rat monoclonal antibody CAMPATH-1 with human leukaemia cells and its possible application for autologous bone marrow transplantation.
    Br J Haematol. 60 (1): 41-8.
  2. Hale, G. et al. (1998) Improving the outcome of bone marrow transplantation by using CD52 monoclonal antibodies to prevent graft-versus-host disease and graft rejection.
    Blood. 92 (12): 4581-90.
  3. Hale, G. et al. (1983) Effects of monoclonal anti-lymphocyte antibodies in vivo in monkeys and humans.
    Mol Biol Med. 1 (3): 321-34.
  4. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.
  5. van der Windt, D.J. et al. (2010) Investigation of lymphocyte depletion and repopulation using alemtuzumab (Campath-1H) in cynomolgus monkeys.
    Am J Transplant. 2010 Apr;10(4):773-83.