CD49c antibody | 17C6
Mouse anti Human CD49c:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 17C6
- Isotype
- IgG1
- Specificity
- CD49c
Antibody Quality and Performance Guarantee
| Mouse anti Human CD49c antibody, clone 17C6 recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c. Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- CD49c transfected cell line.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Cells from immunized Balb/c mice were fused with cells of the NS2 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD49c antibody
-
Nussbaum, G. et al. (2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.
Int Immunol. 18: 1413-9. -
Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
J Cell Biol. 197 (2): 219-30.
- Synonyms
- Integrin Alpha 3 Chain
- VLA-3
- RRID
- AB_322293
- UniProt
- P26006
- Entrez Gene
- ITGA3
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0050900 leukocyte migration
MCA1948F
151404
164447
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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