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CD49c antibody | 17C6

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Anti Human CD49c Antibody, clone 17C6 thumbnail image 1
Anti Human CD49c Antibody, clone 17C6 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B . Alexa Fluor 647 conjugated Mouse anti Human EGFR (MCA1784A647) and PE conjugated Mouse anti Human CD49c (MCA1948PE). All experiments performed on human peripheral blood gated on live single cells, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

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Mouse anti Human CD49c:FITC

Product Type
Monoclonal Antibody
Clone
17C6
Isotype
IgG1
Specificity
CD49c

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Product Code Applications Pack Size List Price Your Price Qty
MCA1948F
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SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Human CD49c antibody, clone 17C6 recognizes CD49c, a ~150 kDa cell surface antigen which is also known as the alpha-3 integrin and as VLA-3. CD49c is very weakly expressed on peripheral blood leucocytes and is not found on platelets. Epithelial cells and endothelial cells express significant levels of CD49c.

Mouse anti Human CD49c antibody, clone 17C6 is routinely tested by flow cytometry on A431 epithelial cell line.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
CD49c transfected cell line.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Cells from immunized Balb/c mice were fused with cells of the NS2 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for CD49c antibody

  1. Nussbaum, G. et al. (2006) Peptide p277 of HSP60 signals T cells: inhibition of inflammatory chemotaxis.
    Int Immunol. 18: 1413-9.
  2. Steinberg, F. et al. (2012) SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways.
    J Cell Biol. 197 (2): 219-30.

Flow Cytometry

Synonyms
Integrin Alpha 3 Chain
VLA-3
RRID
AB_322293
UniProt
P26006
Entrez Gene
ITGA3
GO Terms
GO:0005515 protein binding
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0050900 leukocyte migration

MCA1948F

151404 164447

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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