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CD45RO antibody | UCHL1

Mouse anti Human CD45RO:RPE

Product Type
Monoclonal Antibody
Clone
UCHL1
Isotype
IgG2a
Specificity
CD45RO

Product Code Applications Pack Size List Price Your Price Qty
MCA461PE
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Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.

In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
Marmoset
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Human IL-2 dependent T cells
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3/NS1/1-Ag4-1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests
>
List Price Your Price
Description Mouse IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Smith, S.H. et al. (1986) Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
    Immunology 58: 63-70.

References for CD45RO antibody

  1. Norton, A.J. et al. (1986) Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.
    J Clin Pathol. 39 (4): 399-405.
  2. Terry. L.A. et al. (1987) Phenotypic heterogeneity of the CD4+ and CD8+ subsets.
    Leucocyte Typing III. McMichael, A.J., Beverley, P.C.L. et al. eds. University Press. pp 225-7.
  3. Akbar, A.N. et al. (1988) Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells.
    J Immunol. 140 (7): 2171-8.
  4. Beverley, P.C.L. et al. (1988) Phenotypic diversity of the CD45 antigen and its relation to function.
    Immunology, Suppl. 1: 3-5.
  5. Merkenschlager, M. et al. (1988) Limiting dilution analysis of proliferative responses in human lymphocyte populations defined by the monoclonal antibody UCHL1: implications for differential CD45 expression in T cell memory formation.
    Eur J Immunol. 18 (11): 1653-61.
  6. Terry, L.A. et al. (1988) The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45.
    Immunology. 64 (2): 331-6.
  7. Cavers, M. et al. (2002) Differential expression of beta1 and beta2 integrins and L-selectin on CD4+ and CD8+ T lymphocytes in human blood: comparative analysis between isolated cells, whole blood samples and cryopreserved preparations.
    Clin Exp Immunol. 127: 60-5.
  8. Leigh, J.E. et al. (2006) Characterization of the immune status of CD8+ T cells in oral lesions of human immunodeficiency virus-infected persons with oropharyngeal Candidiasis.
    Clin Vaccine Immunol. 13: 678-83.
  9. View The Latest Product References
  10. Nistala, K. et al. (2008) Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers.
    Arthritis Rheum. 58: 875-87.
  11. Hutnick, N.A. et al. (2010) Vaccination with Ad5 vectors expands Ad5-specific CD8 T cells without altering memory phenotype or functionality.
    PLoS One. 5: e14385.
  12. Liu Y et al. (2015) Fractionation of human spermatogenic cells using STA-PUT gravity sedimentation and their miRNA profiling.
    Sci Rep. 5: 8084.
  13. Wilson, C.L. et al. (2015) Ubiquitin C-terminal hydrolase 1: A novel functional marker for liver myofibroblasts and a therapeutic target in chronic liver disease.
    J Hepatol. 63 (6): 1421-8.

Further Reading

  1. Beverley, P.C. (1987) Human T cell subsets.
    Immunol Lett. 14 (4): 263-7.

RRID
AB_321434
UniProt
P08575
Entrez Gene
PTPRC
GO Terms
GO:0001915 negative regulation of T cell mediated cytotoxicity
GO:0001960 negative regulation of cytokine-mediated signaling pathway
GO:0002378 immunoglobulin biosynthetic process
GO:0005001 transmembrane receptor protein tyrosine phosphatase activity
GO:0005887 integral to plasma membrane
GO:0005925 focal adhesion
GO:0006469 negative regulation of protein kinase activity
GO:0006470 protein dephosphorylation
GO:0007411 axon guidance
GO:0019901 protein kinase binding
GO:0030890 positive regulation of B cell proliferation
GO:0033261 regulation of S phase
GO:0042100 B cell proliferation
GO:0045121 membrane raft
GO:0050852 T cell receptor signaling pathway
GO:0050853 B cell receptor signaling pathway
GO:0050857 positive regulation of antigen receptor-mediated signaling pathway
GO:0051209 release of sequestered calcium ion into cytosol
GO:0051607 defense response to virus

MCA461PE

INN1611

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