CD319 antibody | 162
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F ResetMouse anti Human CD319
- Product Type
- Monoclonal Antibody
- Clone
- 162
- Isotype
- IgG2b
- Specificity
- CD319
Antibody Quality and 
Unrivaled 
| Mouse anti Human CD319 antibody, clone 162 recognizes human CD319, otherwise known as CRACC (CD2-like receptor-activating cytotoxic cells), a type I transmembrane protein and member of the CD2 receptor family, expressed by natural killer (NK) cells, cytotoxic lymphocytes and activated B cells. Unlike the CD2 family receptors 2B4 and NTB-A, which trigger NK cell-mediated cytotoxicity through the recruitment of the adaptor protein SAP (SLAM-associated protein); CD319 has been shown to activate cytotoxicity through a unique SAP-independent ERK-mediated signalling pathway, through association with, and subsequent phosphorylation by, the adaptor protein EAT-2. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CD319 - HuIgG fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2b Negative Control | MCA691 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2b Negative Control | ||||||
References for CD319 antibody
-
Bouchon, A. et al. (2001) Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family.
J Immunol. 167 (10): 5517-21. -
Tassi, I. & Colonna, M. (2005) The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells.
J Immunol. 175 (12): 7996-8002. -
Kawano, Y. et al. (2013) Hypoxia reduces CD138 expression and induces an immature and stem cell-like transcriptional program in myeloma cells.
Int J Oncol. 43 (6): 1809-16. -
Pojero, F. et al. (2016) Utility of CD54, CD229, and CD319 for the identification of plasma cells in patients with clonal plasma cell diseases.
Cytometry B Clin Cytom. 90 (1): 91-100.
- Synonyms
- CRACC
- RRID
- AB_1628806
- UniProt
- Q9NQ25
- Entrez Gene
- SLAMF7
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0042267 natural killer cell mediated cytotoxicity
- GO:0030101 natural killer cell activation
View more products with CD319 specificity
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