CD30 antibody | OTI3B10

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Mouse anti CD30

Mouse anti human CD30 antibody recognizes the tumor necrosis factor receptor superfamily member 8, also known as CD30L receptor, Ki-1 antigen, cytokine receptor CD30 or lymphocyte activation antigen CD30. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI3B10
Isotype
IgG1
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00395 WB 100 µl
Mouse anti Human CD30 antibody recognizes CD30, also known as CD30L receptor, Ki-1 antigen, cytokine receptor CD30, lymphocyte activation antigen CD30 and tumor necrosis factor receptor superfamily member 8 (TNFRSF8).

Encoded by the TNFRSF8 gene, CD30 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of TNFRSF8 encoding distinct isoforms have been reported (provided by RefSeq, Jul 2008).

Mouse anti Human CD30 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti CD30 detects a band of approximately 120 kDa in MOLT-4 cell lysates
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein fragment corresponding to aa 19-379 of human CD30 (NP_001234) produced in E.coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P28908 Related reagents
Entrez Gene
TNFRSF8 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005737 cytoplasm
GO:0008285 negative regulation of cell proliferation
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0043065 positive regulation of apoptosis
GO:0045556 positive regulation of TRAIL biosynthetic process
Acknowledgements
PrecisionAbâ„¢ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD30 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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