CD292 antibody | AbD01564
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WB ResetHuman anti CD292
- Product Type
- Monoclonal Antibody
- Clone
- AbD01564
- Isotype
- HuCAL Fab monovalent
- Specificity
- CD292
Antibody Quality and Performance Guarantee
| Human anti Human CD292 antibody, clone AbD01564 recognizes human CD292, also known as bone morphogenetic protein receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B. The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2.These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding. In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease. Human anti Human CD292 antibody inhibits BMP-2 mediated stimulation of C2C12 cells. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Expected from Sequence Mouse Dog Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Monovalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid.
- Preparation
- Streptactin affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Human BMPR-1A extracellular domain, recombinant expressed in E. coli (amino acid residues 24-152, molecular weight 14.2 kDa)
- Activity
- Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground.
- Purity
- Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample.
- Affinity
- kD = 2.2 nM
The monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain. - Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2ug/ml | |
| Functional Assays | |
||
| Western Blotting | |
2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| Goat anti Human IgG F(ab')2:Alk. Phos. | 0500-0100 | E WB | 0.5 ml |
|
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| List Price | Your Price | ||||||
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|
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| Description | Goat anti Human IgG F(ab')2:Alk. Phos. | ||||||
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
|
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| List Price | Your Price | ||||||
|
|
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| Description | Mouse anti Strep-Tag Classic:HRP | ||||||
References for CD292 antibody
-
Harth, S. et al. (2010) A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.
PLoS One. 5(9). pii: e13049. -
Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 66: 964-8.
Further Reading
-
Katagiri, T. et al. (1994) Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage.
J Cell Biol. 127 (6 Pt 1): 1755-66.
- RRID
- AB_2064257
- UniProt
- P36894
- Entrez Gene
- BMPR1A
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004674 protein serine/threonine kinase activity
- GO:0005024 transforming growth factor beta receptor activity
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006468 protein phosphorylation
- GO:0006955 immune response
- GO:0007179 transforming growth factor beta receptor signaling pathway
- View More GO Terms
- GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
- GO:0030501 positive regulation of bone mineralization
- GO:0030509 BMP signaling pathway
- GO:0042803 protein homodimerization activity
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0046332 SMAD binding
- GO:0060391 positive regulation of SMAD protein import into nucleus
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA004
156110
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