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CD292 antibody | AbD01564

Human anti CD292

Product Type
Monoclonal Antibody
HuCAL Fab monovalent

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
List Price Your Price

Human anti Human CD292 antibody, clone AbD01564 recognizes human CD292, also known as bone morphogenetic protein receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B.

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2.These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding.

In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease. Human anti Human CD292 antibody inhibits BMP-2 mediated stimulation of C2C12 cells.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Monovalent human recombinant Fab (lambda light chain) selected from the HuCAL GOLD phage display library. Expressed in E. coli and purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid.
Streptactin affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% sodium azide (NaN3)
Human BMPR-1A extracellular domain, recombinant expressed in E. coli (amino acid residues 24-152, molecular weight 14.2 kDa)
Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground.
Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample.
kD = 2.2 nM
The monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml
Functional Assays
Western Blotting 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
This antibody contains Strep-tag and will react with streptavidin.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 E WB 0.5 ml loader
List Price Your Price
Description Goat anti Human IgG F(ab')2:HRP
Goat anti Human IgG F(ab')2:Alk. Phos. 0500-0100 E WB 0.5 ml loader
List Price Your Price
Description Goat anti Human IgG F(ab')2:Alk. Phos.
Mouse anti Strep-Tag Classic:HRP MCA2489P E WB 75 µl loader
List Price Your Price
Description Mouse anti Strep-Tag Classic:HRP

References for CD292 antibody

  1. Harth, S. et al. (2010) A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.
    PLoS One. 5(9). pii: e13049.
  2. Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 66: 964-8.

Further Reading

  1. Katagiri, T. et al. (1994) Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage.
    J Cell Biol. 127 (6 Pt 1): 1755-66.

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004674 protein serine/threonine kinase activity
GO:0005024 transforming growth factor beta receptor activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006955 immune response
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030501 positive regulation of bone mineralization
GO:0030509 BMP signaling pathway
GO:0042803 protein homodimerization activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0046332 SMAD binding
GO:0060391 positive regulation of SMAD protein import into nucleus
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.



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