CD292 antibody | AbD01564

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Human anti CD292

Product Type
Monoclonal Antibody
Clone
AbD01564
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA004 E FN WB 0.1 mg
Human anti Human CD292 antibody, clone AbD01564 recognizes human CD292, also known as bone morphogenetic protein receptor type 1A (BMPR-1A) and does not cross-react with human BMPR-1B.

The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR-1A and BMPR-1B and the type II receptor BMPR2.These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. BMPR-1A is the receptor for BMP-2 and BMP-4. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kDa and type II receptors of about 70-80 kDa. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signalling, whereas type I receptors require their respective type II receptors for ligand binding.

In humans, mutations in the BMPR-1A gene are responsible for juvenile polyposis syndrome, juvenile intestinal polyposis, and Cowden disease. Human anti Human CD292 antibody inhibits BMP-2 mediated stimulation of C2C12 cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Mouse
Dog Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using Streptactin affinity chromatography. The antibody is tagged with a strepII-tag (NWSHPQFEK) at the C-terminus of the antibody heavy chain-liquid.
Preparation
Streptactin affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Human BMPR-1A extracellular domain, recombinant expressed in E. coli (amino acid residues 24-152, molecular weight 14.2 kDa)
Activity
Activity was tested by indirect ELISA: recombinant purified BMPR-1A (5 μg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA004 (2 μg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate, 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above back-ground.
Purity
Purity was tested by SDS-PAGE and Coomassie-staining of a 2 μg sample.
Affinity
kD = 2.2 nM
The monovalent intrinsic affinity of HCA004 was measured by BIAcore on the immobilized BMPR-1A extracellular domain.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P36894 Related reagents
Entrez Gene
BMPR1A Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004674 protein serine/threonine kinase activity
GO:0005024 transforming growth factor beta receptor activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0006955 immune response
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0030501 positive regulation of bone mineralization
GO:0030509 BMP signaling pathway
GO:0042803 protein homodimerization activity
GO:0045669 positive regulation of osteoblast differentiation
GO:0046332 SMAD binding
GO:0060391 positive regulation of SMAD protein import into nucleus
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Blot data kindly provided by Professor Sebald, University of Wuerzburg, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CD292 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2ug/ml
Functional Assays
Western Blotting 2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Goat anti Human IgG F(ab')2:Alk. Phos. 0500-0100 0.5 ml E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Product Specific References

References for CD292 antibody

  1. Harth, S. et al. (2010) A selection fit mechanism in BMP receptor IA as a possible source for BMP ligand-receptor promiscuity.
    PLoS One. 5(9). pii: e13049.
  2. Harth, S. et al. (2010) Crystallization of BMP receptor type IA bound to the antibody Fab fragment AbD1556.
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 66: 964-8.

Further Reading

  1. Katagiri, T. et al. (1994) Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage.
    J Cell Biol. 127 (6 Pt 1): 1755-66.