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CD284 antibody | HTA125

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Anti Human CD284 Antibody, clone HTA125 thumbnail image 1 Anti Human CD284 Antibody, clone HTA125 thumbnail image 2 Anti Human CD284 Antibody, clone HTA125 thumbnail image 3 Anti Human CD284 Antibody, clone HTA125 thumbnail image 4 Anti Human CD284 Antibody, clone HTA125 thumbnail image 5 Anti Human CD284 Antibody, clone HTA125 thumbnail image 6 Anti Human CD284 Antibody, clone HTA125 thumbnail image 7 Anti Human CD284 Antibody, clone HTA125 thumbnail image 8
Anti Human CD284 Antibody, clone HTA125 gallery image 1
Staining of human peripheral blood monocytes with Mouse anti Human CD284, clone HTA125 (MCA2061).
Anti Human CD284 Antibody, clone HTA125 gallery image 2
Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor® 488 (MCA2061A488).
Anti Human CD284 Antibody, clone HTA125 gallery image 3
Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor® 647 (MCA2061A647).
Anti Human CD284 Antibody, clone HTA125 gallery image 4
Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC (MCA2061F).
Anti Human CD284 Antibody, clone HTA125 gallery image 5
Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin (MCA2061EL).
Anti Human CD284 Antibody, clone HTA125 gallery image 6
Staining of U937 cells with Mouse anti Human CD284:RPE (MCA2061PE).
Anti Human CD284 Antibody, clone HTA125 gallery image 7
Published customer image:
Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL) used for the evaluation of TLR4 blockade on the phagocytic activity of neutrophils in whole blood
Image caption:
Influence of antibodies against TLR4, CD14 and CD11b on neutrophil phagocytic activity in whole blood. Control cells without any incentives (C), cells activated by S-LPS (C1) and cells activated by Re-LPS (C2).

From: Prokhorenko, I. et al.
Toll-like receptor 4 in phagocytosis of Escherichia coli by endotoxin-activated human neutrophils in whole blood.
Critical Care201216(Suppl 3):P80
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD284 Antibody, clone HTA125 gallery image 8
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD14 (​MCA1568SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD14 (​MCA1568SBV440) and StarBright Violet 790 conjugated Mouse anti Human CD284 (​MCA2061SBV790). All experiments performed on human PBMCs gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Mouse anti Human CD284:StarBright Violet 790

Product Type
Monoclonal Antibody
Clone
HTA125
Isotype
IgG2a
Specificity
CD284

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Product Code Applications Pack Size List Price Your Price Qty
MCA2061SBV790
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
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Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.

TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.

TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.

This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL ) is recommended for functional assays.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Guinea Pig
Pig
Dog
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to StarBright Violet 790 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Ba/F3 cell line expressing TLR4 (CD284).
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 790 402 782
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD284 antibody

  1. Shimazu, R. et al. (1999) MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.
    J Exp Med. 189 (11): 1777-82.
  2. Sugawara, S. et al. (2000) Proteolysis of human monocyte CD14 by cysteine proteinases (gingipains) from Porphyromonas gingivalis leading to lipopolysaccharide hyporesponsiveness.
    J Immunol. 165: 411-8.
  3. Yang, S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture.
    Infect Immun. 69 (4): 2045-53.
  4. Kawahara T et al. (2001) Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.
    Infect Immun. 69 (7): 4382-9.
  5. Baumgarten, G. et al. (2001) In vivo expression of proinflammatory mediators in the adult heart after endotoxin administration: the role of toll-like receptor-4.
    J Infect Dis. 183: 1617-24.
  6. Medvedev, A.E. et al. (2001) Induction of tolerance to lipopolysaccharide and mycobacterial components in Chinese hamster ovary/CD14 cells is not affected by overexpression of Toll-like receptors 2 or 4.
    J Immunol. 167: 2257-67.
  7. Bieback, K. et al. (2002) Hemagglutinin protein of wild-type measles virus activates toll-like receptor 2 signaling.
    J Virol. 76: 8729-36.
  8. Karlsson, H. et al. (2002) Innate immune responses of human neonatal cells to bacteria from the normal gastrointestinal flora.
    Infect Immun. 70: 6688-96.
    9. View The Latest Product References
  9. Devaney, J.M. (2003) Neutrophil elastase up-regulates interleukin-8 via toll-like receptor 4.
    FEBS Lett. 544:129-32.
  10. Elner, S.G. et al. (2005) TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression.
    Invest Ophthalmol Vis Sci. 46 (12): 4627-33.
  11. Cuschieri, J. et al. (2006) Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activation.
    J Leukoc Biol. 80: 1289-97.
  12. Lindsay, J.O. et al. (2006) Clinical, microbiological, and immunological effects of fructo-oligosaccharide in patients with Crohn's disease.
    Gut. 55: 348-55.
  13. Foster, N. et al. (2007) Pivotal advance: vasoactive intestinal peptide inhibits up-regulation of human monocyte TLR2 and TLR4 by LPS and differentiation of monocytes to macrophages.
    J Leukoc Biol. 81: 893-903.
  14. Pioli, P.A. et al. (2007) Estradiol attenuates lipopolysaccharide-induced CXC chemokine ligand 8 production by human peripheral blood monocytes.
    J Immunol. 179: 6284-90.
  15. Ibeagha-Awemu, E.M. et al. (2008) Bacterial lipopolysaccharide induces increased expression of toll-like receptor (TLR) 4 and downstream TLR signaling molecules in bovine mammary epithelial cells.
    Vet Res. 39 (2): 11.
  16. Chochi, K. et al. (2008) Helicobacter pylori augments growth of gastric cancers via the lipopolysaccharide-toll-like receptor 4 pathway whereas its lipopolysaccharide attenuates antitumor activities of human mononuclear cells.
    Clin Cancer Res. 14 (10): 2909-17.
  17. Brännström, K. et al. (2009) The Schistosoma mansoni protein Sm16/SmSLP/SmSPO-1 assembles into a nine-subunit oligomer with potential To inhibit Toll-like receptor signaling.
    Infect Immun. 77: 1144-54.
  18. de Kleer, I. (2010) CD30 Discriminates Heat Shock Protein 60-Induced FOXP3+CD4+ T Cells with a Regulatory Phenotype.
    J Immunol. 185(4):2071-9.
  19. Garbe, K. et al. (2012) Plasmacytoid dendritic cells and their Toll-like receptor 9 expression selectively decrease with age.
    Hum Immunol. 73 (5): 493-7.
  20. Komori, H. et al. (2012) α(1)-Acid glycoprotein up-regulates CD163 via TLR4/CD14 protein pathway: possible protection against hemolysis-induced oxidative stress.
    J Biol Chem. 287 (36): 30688-700.
  21. Prokhorenko, I. et al. (2012) Toll-like receptor 4 in phagocytosis of Escherichia coli by endotoxin-activated human neutrophils in whole blood
    Crit Care 16 (Suppl 3), P80
  22. Sels, J.W. et al. (2012) Fractional flow reserve is not associated with inflammatory markers in patients with stable coronary artery disease.
    PLoS One. 7: e46356.
  23. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
  24. Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.
  25. Zwolak, A. et al. (2015) Hyperreactivity of Blood Leukocytes in Patients with NAFLD to ex vivo Lipopolysaccharide Treatment Is Modulated by Metformin and Phosphatidylcholine but Not by Alpha Ketoglutarate.
    PLoS One. 10 (12): e0143851.
  26. Xu, H. et al. (2015) Type 3 innate lymphoid cell depletion is mediated by TLRs in lymphoid tissues of simian immunodeficiency virus-infected macaques.
    FASEB J. 29 (12): 5072-80.
  27. Blagitz, M,G. et al. (2015) Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis
    Pesquisa Veterinária Brasileira. 35 (1): 1-5.
  28. Mazzucchelli, I. et al. (2015) Expression and function of toll-like receptors in human circulating endothelial colony forming cells.
    Immunol Lett. 168 (1): 98-104.
  29. Zwolak, A. et al. (2016) Metformin Changes the Relationship between Blood Monocyte Toll-Like Receptor 4 Levels and Nonalcoholic Fatty Liver Disease-Ex Vivo Studies.
    PLoS One. 11 (3): e0150233.
  30. Huang, D. et al. (2016) Hyperoxia induces inflammation and regulates cytokine production in alveolar epithelium through TLR2/4-NF-κB-dependent mechanism
    Eur Rev Med Pharmacol Sci. 20: 1399-410.
  31. Reineking, W. et al. (2018) Canine primary jejunal and colonic epithelial cells predominantly express TLR5 and TLR9 but do not change TLR expression pattern after stimulation with certain Toll-like receptor ligands.
    Vet Immunol Immunopathol. 206: 16-24.
  32. Awuah, D. et al. (2019) The Cross-Talk between miR-511-3p and C-Type Lectin Receptors on Dendritic Cells Affects Dendritic Cell Function.
    J Immunol. 203 (1): 148-57.

Flow Cytometry

Functional Assays

Western Blotting

Synonyms
TLR4
UniProt
O00206
Entrez Gene
TLR4

MCA2061SBV790

64595341

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