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CD28 antibody | YTH913.12

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Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 1 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 2 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 3 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 4 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 5 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 6 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 7 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 8 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 9 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 10 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 11 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 12 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 13 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 14 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 15 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 16 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 17 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 18 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 19 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 20 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 21 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 22 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 23 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 24 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 25 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 26 Anti Human CD28 Antibody, clone YTH913.12 thumbnail image 27
Anti Human CD28 Antibody, clone YTH913.12 gallery image 1
Figure A. Alexa Fluor647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated rat IgG2b isotype control (MCA6006PE). Figure B. Alexa Fluor647 conjugated mouse anti human CD4 (MCA1267A647) and RPE conjugated rat anti human CD28 (MCA709PE). All experiments performed on human Peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Anti Human CD28 Antibody, clone YTH913.12 gallery image 2
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A488) and SBV515 conjugated Rat anti Human CD28 (MCA709SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 3
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Violet 670 conjugated Rat anti Human CD28 (MCA709SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 4
Figure A. Alexa Fluor A488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor A488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 440 conjugated Rat anti Human CD28 (MCA709SBV440). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 5
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 610 conjugated Rat anti Human CD28 (MCA709SBV610). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 6
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Violet 570 conjugated Rat anti Human CD28 (MCA709SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 7
Figure A. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700). Figure B. Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and StarBright Violet 710 conjugated Rat anti Human CD28 (MCA709SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 8
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 790 conjugated Rat anti Human CD28 (MCA709SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 9
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright Violet 475 conjugated Rat anti Human CD28 (MCA709SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 10
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright UltraViolet 400 conjugated Rat anti Human CD28 (MCA709SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 11
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Violet 760 conjugated Mouse anti Human CD28 (MCA709SBV760). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 12
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and StarBright UltraViolet 665 conjugated Rat anti Human CD28 (MCA709SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 13
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright UltraViolet 445 conjugated Mouse anti Human CD28 (MCA709SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 14
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright UltraViolet 795 conjugated Rat anti human CD28 (MCA709SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 15
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright UltraViolet 575 conjugated Rat anti Human CD28 (MCA709SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 16
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and StarBright UltraViolet 605 conjugated Rat anti Human CD28 (MCA709SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 17
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 580 conjugated Rat anti Human CD28 (MCA709SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 18
Figure A. StarBright Violet 475 conjugated Mouse anti Human CD3 (MCA463SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Human CD3 (MCA463SBV475) and StarBright Blue 615 conjugated Rat anti Human CD28 (MCA709SBB615). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 19
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Blue 675 conjugated Rat anti Human CD28 (MCA709SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 20
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD3 (MCA463SBV440) and StarBright Blue 765 conjugated Mouse anti Human CD28 (MCA709SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 21
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD3 (MCA463SBV515) and StarBright Blue 810 conjugated Rat anti Human CD28 (MCA709SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 22
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and StarBright Yellow 575 conjugated Rat anti Human CD28 (MCA709SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 23
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright Yellow 720 conjugated Mouse anti Human CD28 (MCA709SBY720). All experiments performed on human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 24
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD3 (MCA463SBV790) and StarBright Yellow 665 conjugated Rat anti Human CD28 (MCA709SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 25
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD3 (MCA463A647) and StarBright Yellow 800 conjugated Mouse anti Human CD28 (MCA709SBY800). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 26
Figure A. StarBright Blue765 conjugated Mouse anti Human CD3 (MCA463SBB765). Figure B. StarBright Blue765 conjugated Mouse anti Human CD3 (MCA463SBB765) and StarBright Yellow 605 conjugated Mouse anti Human CD28 (MCA709SBY605). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD28 Antibody, clone YTH913.12 gallery image 27
Figure A. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610). Figure B. StarBright Violet 610 conjugated Mouse anti Human CD3 (MCA463SBV610) and StarBright Red 775 conjugated Mouse anti Human CD28 (MCA709SBR775). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Rat anti Human CD28:StarBright Yellow 800

Product Type
Monoclonal Antibody
Clone
YTH913.12
Isotype
IgG2b
Specificity
CD28

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Product Code Applications Pack Size List Price Your Price Qty
MCA709SBY800
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
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Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44 kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Viola et al.1996).

CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummel et al. 1995).

Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived NK cells using clone YTH913.12 (Wilson et al. 1999).

Target Species
Human
Product Form
Purified IgG conjugated to StarBright Yellow 800 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Human peripheral blood T-cells.
Fusion Partners
Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Yellow 800 549 788
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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References for CD28 antibody

  1. Reiter, C. (1989) Cluster Report: CD28 in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W., Dorken, B., Gilks, W.R., Rieber, E.P., Schmidt, R.E., Stein, H. and von dem Borne, A.E.G.Kr.
    Oxford University Press. pp 352-3.
  2. McLeod, J.D. et al. (1998) Activation of human T cells with superantigen (staphylococcal enterotoxin B) and CD28 confers resistance to apoptosis via CD95.
    J Immunol. 160: 2072-9.
  3. Galea-Lauri, J. et al. (1999) Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells.
    J Immunol. 163 (1): 62-70.
  4. Wilson, J.L. et al. (1999) NK cell triggering by the human costimulatory molecules CD80 and CD86.
    J Immunol. 163: 4207-12.
  5. Costa, C. et al. (2002) Human NK cell-mediated cytotoxicity triggered by CD86 and Gal alpha 1,3-Gal is inhibited in genetically modified porcine cells.
    J Immunol. 168: 3808-16.
  6. Ponchel, F. et al. (2002) Dysregulated lymphocyte proliferation and differentiation in patients with rheumatoid arthritis.
    Blood. 100: 4550-6.
  7. Blanco, B. et al. (2003) Induction of human T lymphocyte cytotoxicity and inhibition of tumor growth by tumor-specific diabody-based molecules secreted from gene-modified bystander cells.
    J Immunol. 171: 1070-7.
  8. Johnston, A. et al. (2004) Peripheral blood T cell responses to keratin peptides that share sequences with streptococcal M proteins are largely restricted to skin-homing CD8(+) T cells.
    Clin Exp Immunol. 138 (1): 83-93.
    9. View The Latest Product References
  9. Goodier, M.R. and Londei, M. (2004) CD28 is not directly involved in the response of human CD3- CD56+ natural killer cells to lipopolysaccharide: a role for T cells.
    Immunology. 111: 384-90.
  10. Kropf, P. et al. (2007) Arginase activity mediates reversible T cell hyporesponsiveness in human pregnancy.
    Eur J Immunol. 37: 935-45.
  11. Gabdoulkhakova, A. et al. (2007) High rate of mutation reporter gene inactivation during human T cell proliferation.
    Immunogenetics. 59: 135-43.
  12. Pridgeon, C. et al. (2011) Regulation of IL-17 in chronic inflammation in the human lung.
    Clin Sci (Lond). 120: 515-24.
  13. Litjens, N.H. et al. (2011) Identification of Circulating Human Antigen-Reactive CD4+FOXP3+ Natural Regulatory T Cells.
    J Immunol. 188: 1083-90.
  14. Svensson-Arvelund, J. et al. (2015) The human fetal placenta promotes tolerance against the semiallogeneic fetus by inducing regulatory T cells and homeostatic M2 macrophages.
    J Immunol. 194 (4): 1534-44.
  15. Hasib, L. et al. (2016) Functional and homeostatic defects of regulatory T cells in patients with coronary artery disease.
    J Intern Med. 279 (1): 63-77.
  16. Siska, E.K. et al. (2017) Generation of an immortalized mesenchymal stem cell line producing a secreted biosensor protein for glucose monitoring.
    PLoS One. 12 (9): e0185498.
  17. Hellberg, S. et al. (2021) Progesterone Dampens Immune Responses in In Vitro Activated CD4+ T Cells and Affects Genes Associated With Autoimmune Diseases That Improve During Pregnancy.
    Front Immunol. 12: 672168.
  18. Zenere, A. et al. (2023) Prominent epigenetic and transcriptomic changes in CD4(+) and CD8(+) T cells during and after pregnancy in women with multiple sclerosis and controls.
    J Neuroinflammation. 20 (1): 98.

Flow Cytometry

Functional Assays

UniProt
P10747
Entrez Gene
CD28

MCA709SBY800

100007758

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