CD279 antibody | EH12.2H7

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Mouse anti Human CD279:StarBright UltraViolet 540

Product Type: Monoclonal Antibody
Clone: EH12.2H7
Isotype: IgG1
Specificity: CD279

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MCA6133SBUV540 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human CD279 Antibody, clone EH12.2H7 thumbnail image 1

Anti Human CD279 Antibody, clone EH12.2H7 thumbnail image 2

Anti Human CD279 Antibody, clone EH12.2H7 thumbnail image 3

Anti Human CD279 Antibody, clone EH12.2H7 thumbnail image 4

Anti Human CD279 Antibody, clone EH12.2H7 thumbnail image 5

Anti Human CD279 Antibody, clone EH12.2H7 gallery image 1 — Surface staining of PHA-activated human PBMCs with Mouse anti Human CD279:RPE (MCA6133PE)

Anti Human CD279 Antibody, clone EH12.2H7 gallery image 2 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) and Anti-Human CD279 StarBright UltraViolet 540 (**MCA6133SBUV540**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD279 Antibody, clone EH12.2H7 gallery image 3 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) and Anti-Human CD279 StarBright UltraViolet 700 (**MCA6133SBUV700**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD279 Antibody, clone EH12.2H7 gallery image 4 — Human PBMCs was stained with Anti-Human CD3 StarBright UltraViolet 400 (**MCA463SBUV400**) only (Figure A) or with with Anti-Human CD3 StarBright UltraViolet 400 (**MCA463SBUV400**) and Anti-Human CD279 StarBright UltraViolet 770 (**MCA6133SBUV770**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA for 72-hours, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD279 Antibody, clone EH12.2H7 gallery image 5 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 790 (**MCA463SBV790**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 790 (**MCA463SBV790**) and Anti-Human CD279 StarBright Violet 535 (**MCA6133SBV535**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA for 72-hours, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

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Mouse anti Human CD279 antibody, clone EH12.2H7 detects CD279, a co-stimulatory molecule also known as programmed cell death-1 (PD-1).

CD279 is a ~50-55 kDa membrane protein which is a member of the CD28 family, and functions as a regulator of T cell activation (Fife and Pauken, 2011). It has two specific ligands; CD274 (PD-L1) and CD273 (PD-L2), and their interaction is key in the balance between stimulatory and inhibitory signals needed for effective immune responses to microbes and self-tolerance.

CD279 is a marker for germinal center-associated T cells (Dorfman et al, 2006) and is inducibly expressed by T cells, B cells, NKT cells and monocytes upon activation.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Primate; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to StarBright UltraViolet 540 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright UltraViolet 540 338 540
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Human Seroblock	BUF070A	F	50 Test	Log in
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Description	Human Seroblock
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Description	Human Seroblock

Synonyms: PD-1
UniProt: Q15116
Entrez Gene: PDCD1
GO Terms: GO:0006915 apoptosis; GO:0016021 integral to membrane; GO:0004725 protein tyrosine phosphatase activity; GO:0004871 signal transducer activity; GO:0006959 humoral immune response; GO:0007275 multicellular organismal development; GO:0031295 T cell costimulation