CD274 antibody | RM320

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Rabbit anti CD274

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 ml loader

Rabbit anti Human CD274 antibody recognizes programmed cell death 1 ligand 1 (PD-L1), also known as B7-H1. CD274 is a cell-surface glycoprotein induced by cytokines, importantly interferon gamma from T helper cells under inflammatory conditions (Inaguma et al. 2017). CD274 is expressed in a range of tissues, but mostly in activated T and B lymphocyte cells, dendritic cells, and monocytes (Fabrizio et al. 2018). CD274 is an inhibitory immune checkpoint inhibitor which plays a role in maintaining peripheral T-lymphocyte tolerance and regulating inflammation (Schutz et al. 2017). Constitutive upregulation of CD274 has been identified in solid tumors (Franzen et al. 2017), and expression of CD274 in the tumor microenvironment appears to be one of the main mechanisms in which cancer can evade the immune system via inhibiting anti-tumor T-cell immunity (Zou et al. 2016).

Product Details

Target Species
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol
A peptide corresponding to the C-terminus of Human CD274

Storage Information

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0042130 negative regulation of T cell proliferation
For research purposes only

Applications of CD274 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/200 1/500
Immunohistology - Paraffin 1/200 1/1000
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader

Fluorescent Spectraviewer

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How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra