Rabbit anti Human CD274 antibody recognizes programmed cell death 1 ligand 1 (PD-L1), also known as B7-H1. CD274 is a cell-surface glycoprotein induced by cytokines, importantly interferon gamma from T helper cells under inflammatory conditions (Inaguma et al. 2017). CD274 is expressed in a range of tissues, but mostly in activated T and B lymphocyte cells, dendritic cells, and monocytes (Fabrizio et al. 2018). CD274 is an inhibitory immune checkpoint inhibitor which plays a role in maintaining peripheral T-lymphocyte tolerance and regulating inflammation (Schutz et al. 2017). Constitutive upregulation of CD274 has been identified in solid tumors (Franzen et al. 2017), and expression of CD274 in the tumor microenvironment appears to be one of the main mechanisms in which cancer can evade the immune system via inhibiting anti-tumor T-cell immunity (Zou et al. 2016).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography of Protein A from animal origin-free culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin
- A peptide corresponding to the C-terminus of Human CD274
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
external side of plasma membrane
negative regulation of T cell proliferation
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD274 antibody
|Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
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