CD274 antibody | 29E.2A3

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Mouse anti Human CD274:StarBright Violet 535

Product Type: Monoclonal Antibody
Clone: 29E.2A3
Isotype: IgG2b
Specificity: CD274

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MCA6132SBV535 Datasheet SDS SDS	F	100 Tests/0.5ml		Log in
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Anti Human CD274 Antibody, clone 29E.2A3 thumbnail image 1

Anti Human CD274 Antibody, clone 29E.2A3 thumbnail image 2

Anti Human CD274 Antibody, clone 29E.2A3 thumbnail image 3

Anti Human CD274 Antibody, clone 29E.2A3 thumbnail image 4

Anti Human CD274 Antibody, clone 29E.2A3 thumbnail image 5

Anti Human CD274 Antibody, clone 29E.2A3 gallery image 1 — Surface staining of PHA-stimulated PBMCs with Mouse anti Human CD274:RPE (MCA6132PE)

Anti Human CD274 Antibody, clone 29E.2A3 gallery image 2 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) and Anti-Human CD274 StarBright UltraViolet 540 (**MCA6132SBUV540**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD274 Antibody, clone 29E.2A3 gallery image 3 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 440 (**MCA463SBV440**) and Anti-Human CD274 StarBright UltraViolet 700 (**MCA6132SBUV700**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD274 Antibody, clone 29E.2A3 gallery image 4 — Human PBMCs was stained with Anti-Human CD3 StarBright UltraViolet 400 (**MCA463SBUV400**) only (Figure A) or with with Anti-Human CD3 StarBright UltraViolet 400 (**MCA463SBUV400**) and Anti-Human CD274 StarBright UltraViolet 770 (**MCA6132SBUV770**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA for 72-hours, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

Anti Human CD274 Antibody, clone 29E.2A3 gallery image 5 — Human PBMCs was stained with Anti-Human CD3 StarBright Violet 790 (**MCA463SBV790**) only (Figure A) or with with Anti-Human CD3 StarBright Violet 790 (**MCA463SBV790**) and Anti-Human CD274 StarBright Violet 535 (**MCA6132SBV535**) (Figure B). Experiments were performed on PBMCs extracted from human blood stimulated with PHA for 72-hours, gated on live, single Lymphocytes. Data were acquired on a Cytek Aurora.

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Mouse anti Human CD274 antibody, clone 29E.2A3 detects human CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T cells, B cells, endothelial cells and epithelial cells in response to interferons alpha, beta and gamma.

CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naïve T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to co-stimulate early T cell priming and differentiation.

Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Primate; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to StarBright Violet 535 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Full length human CD274
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Violet 535 402 540
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 10⁶ cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

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Human Seroblock	BUF070A	F	50 Test	Log in
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Description	Human Seroblock
Human Seroblock	BUF070B	F	200 Test	Log in
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Description	Human Seroblock

Synonyms: PD-L1
UniProt: Q9NZQ7
Entrez Gene: CD274
GO Terms: GO:0016021 integral to membrane; GO:0004872 receptor activity; GO:0006955 immune response; GO:0007166 cell surface receptor linked signaling pathway; GO:0008283 cell proliferation; GO:0012505 endomembrane system; GO:0031295 T cell costimulation